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Problem Set 1

This document contains a problem set for a biochemical engineering course. It includes 4 problems related to modeling enzyme kinetics and reaction rates using Michaelis-Menten equations. Problem 1 involves deriving equations to calculate reaction time and substrate concentration for a modified rate equation. Problem 2 asks students to derive an equation to calculate reactor volume for a batch process. Problem 3 involves modeling substrate and product concentration over time using Michaelis-Menten kinetics. Problem 4 asks students to determine kinetic parameters from experimental rate data and calculate turnover number.

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Ken Venzon
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122 views

Problem Set 1

This document contains a problem set for a biochemical engineering course. It includes 4 problems related to modeling enzyme kinetics and reaction rates using Michaelis-Menten equations. Problem 1 involves deriving equations to calculate reaction time and substrate concentration for a modified rate equation. Problem 2 asks students to derive an equation to calculate reactor volume for a batch process. Problem 3 involves modeling substrate and product concentration over time using Michaelis-Menten kinetics. Problem 4 asks students to determine kinetic parameters from experimental rate data and calculate turnover number.

Uploaded by

Ken Venzon
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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PROBLEM SET 1

CHEP 533: Biochemical Engineering


Group Members:

Due Date: November 13, 2015

____________________________
____________________________
____________________________

1. A modified reaction velocity or rate equation below instead of the Michaelis-Menten (M-M) model is used to
predict conversion in a batch reactor. The initial substrate concentration is 10-5uM, km = 2x10-3 uM and vm =
6.77x10-7 uM/s with value of ks = 0.1uM as constant;
vm .[S ]
v=
[S ]2
'
k m + [S ] +
KS
a. Derive the new equation for calculating the time for reaction.
b. How long (in min.) shall I run the reaction to obtain an 80% conversion?
c. Which substrate concentration in this given set gives the highest reaction velocity?
(S in uM = 0.08, 0.02, 0.014, 0.0044, 5x10-4)
d. Plot the v vs. [S] graph. Comment on the result.
2.

Derive an equation to calculate the volume of a reactor in a batch process using M-M model.

3.

An enzyme with km = 2.6x10-3M was assayed at an initial substrate concentration of 0.3M. The observed velocity was
5.9x10-5M/min. If the initial concentration of substrate is 2x10-5M,
a. what would be the product concentration after 90 min.?
b. determine the yield for 90 min.
c. construct a plot of the concentration of substrate and product with time for 5 data points (1, 10, 30, 50, 70,
90 min.)
d. to obtain a 98% conversion, how long shall I wait for my reaction to achieve such conversion?
e. comment on the order of the reaction

4. You are given a 4.0 ug/mL solution of an enzyme (MW = 40,000Da). In your experiment, you have determined
the initial reaction velocities vo at several substrate concentrations, [S].
a. Determine the Km and Vm using lineweaver-Burke Plot
b. Calculate the turnover number for the reaction
S (mM)
0.25
0.4
0.7
1
2
3
5
8
12
20

V0 (mM/min)
0.06
0.08
0.13
0.16
0.25
0.31
0.36
0.39
0.43
0.46

------------------------------------------- END ------------------------------------------"I pledge on my honor that as the group representative, I have honestly accomplished this homework and assignment only with the
help of my group mates and that sharing our solutions, answers and ideas to other groups/individuals violates the principle of
independent thinking and working which constitutes our core integrity as students of CHEP 533 Biochemical Engineering. I believe
that doing my best effort with the right mindset and attitude will fulfill my highest ideals as a student of this institution."

____________________________________
Signature of group representative

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