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Tugas TLC MHS

The document discusses analyzing compounds using thin layer chromatography (TLC). It includes questions about determining Rf values of compounds A, B, and C run on silica gel TLC plates using hexanes as the solvent. It also asks how the Rf values would change using different solvents or plate materials. Another question asks how to modify the solvent system to better separate compounds X, Y, and Z. Finally, it discusses analyzing an unknown sample by TLC and determining purity based on the results.

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Cipoxz Paricuk
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95 views

Tugas TLC MHS

The document discusses analyzing compounds using thin layer chromatography (TLC). It includes questions about determining Rf values of compounds A, B, and C run on silica gel TLC plates using hexanes as the solvent. It also asks how the Rf values would change using different solvents or plate materials. Another question asks how to modify the solvent system to better separate compounds X, Y, and Z. Finally, it discusses analyzing an unknown sample by TLC and determining purity based on the results.

Uploaded by

Cipoxz Paricuk
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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TUGAS ANALISIS KROMATOGRAFI

1) Consider the following silica gel TLC plate of compounds A, B, and C developed in
hexanes:

a) Determine the R f values of compounds A, B, and C run on a silica gel TLC


plate using hexanes as the solvent
b) Which compound, A, B, or C, is the most polar?
c) What would you expect to happen to the R f values if you used acetone instead
of hexanes as the eluting solvent?
d) How would the R f values change if eluted with hexanes using an alumina TLC
plate?
2) You are trying to determine a TLC solvent system which will separate the compounds
X, Y, and Z. You ran the compounds on a TLC plate using hexanes/ethyl acetate 95:5 as
the eluting solvent and obtained the chromatogram below. How could you change the
solvent system to give better separation of these three compounds?

3) After a rather lengthy organic chemistry synthesis procedure, a student ran the product
of the reaction on a TLC plate and obtained the result below. What might he/she have
done wrong, if anything?

4) A student spots an unknown sample on a TLC plate. After developing in hexanes/ethyl


acetate 50:50, he/she saw a single spot with an R f of 0.55. Does this indicate that the
unknown material is a pure compound? What can be done to verify the purity of the
sample?
5) Consider a sample that is a mixture composed of biphenyl, benzoic acid, and benzyl
alcohol. The sample is spotted on a TLC plate and developed in a hexanes/ethyl acetate
solvent mixture. Predict the relative R f values for the three compounds in the sample.

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