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Calibration Curve: Tartaric Acid: Peak Area

The document contains calibration curves and data for the analysis of tartaric acid, malic acid, lactic acid, citric acid, and caffeine using UV-Vis spectroscopy. Linear regression was used to analyze the calibration curves and determine the linear equation and R2 value for each compound.

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janesalazar3369
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51 views

Calibration Curve: Tartaric Acid: Peak Area

The document contains calibration curves and data for the analysis of tartaric acid, malic acid, lactic acid, citric acid, and caffeine using UV-Vis spectroscopy. Linear regression was used to analyze the calibration curves and determine the linear equation and R2 value for each compound.

Uploaded by

janesalazar3369
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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Tartaric Acid

V1

Molarity1
0.25
0.5
2.5
4
5

Tartaric Acid
y

Concentration
50

m
550
548
555

Peak Area
0.5
1
5
8
10

b
393.81

8.9074

Ave.

169
406
1943
3407
3768

4500
4000
3500
3000
2500
Peak Area
2000
1500
1000
500
0

Calculated X
1.373994058
1.368915467
1.386690536

1.376533354 1.377

For SD
X-MEAN
6.44802E-06
5.80322E-05
0.000103168

Calibration Cu

0.009

Summation
SD
0.000167649
0.00915556

Calibration Curve: Tartaric Acid


y = 393.81x + 8.9074
R = 0.9913
Series1
Linear (Series1)

10

Concentration (g/L)

15

Malic Acid
V1

Molarity1
0.1
0.5
2.5
5
7.5

Concentration
50

m
160
150
157

Peak Area
0.2
1
5
10
15

b
131.84

-2.0669

Ave.

54
130
622
1298
1999

Calculated X
1.229269569
1.153420055
1.206514715
1.196401446

For SD
X-MEAN
0.001080314
0.0018474
0.000102278

Summation
0.003029992

Calibration Curve: Malic Acid


2500
y = 131.84x - 2.0669
R = 0.9989

2000
1500
Peak Area

Series1

1000

Linear (Series1)

500
0
0

10
Concentration (g/L)

1.196

0.039

SD
0.038922948

15

20

Lactic acid
V1

Molarity1
0.1
1
2.5
3
5

Concentration
25

Calibration C

Peak Area
0.1
1
2.5
3
5

29
284
739
909
1364

1600
1400
1200
1000

Peak Area 800


600
400
200

m
1000
997
990

b
276.51

Calculated X
23.491
3.531550396
3.520700879
3.495385339

Ave.

3.515878871 3.516

For SD
X-MEAN
0.000245597
2.32518E-05
0.000419985

0
0

0.019

Summation
SD
0.000688833 0.018558466

Calibration Curve: Lactic Acid


y = 276.51x + 23.491
R = 0.9943

Series1
Linear (Series1)

Concentration (g/L)

Citric acid
V1
Molarity1
Concentration
Peak Area
0.25
5
0.05
0.5
0.1
2.5
0.5
3.75
0.75
5
1

m
190
188
181

b
407.59

-6.0446

Ave.

Calibration Curve:

16
38
181
315
398

450
400
350
300
250
Peak Area
200
150
100
50
0

Calculated X
0.480984813
0.476077921
0.4589038

0.471988845 0.472

For SD
X-MEAN
Summation
SD
8.09274E-05
0.000268866
1.67205E-05
0.000171218

0.5

Concentration (g/L)

0.012

0.011594533

alibration Curve: Citric Acid


y = 407.59x - 6.0446
R = 0.9952
Series1
Linear (Series1)
Linear (Series1)

0.5

Concentration (g/L)

1.5

Concentration

Area
50
100
150
200
250

Unknown

Retention Time
1130122
1931827
2647102
3342982
3926552

m
3387848
3456374
3564523

b
14008

494513

Ave.
For SD
X-MEAN
34.04471465
0.888996259
45.93655126

RT
2.242
2.268
2.246
2.269

Calibration

2.251
2.241
2.241
2.237
2.236

Calculated X
206.5487579
211.4406768
219.1611936
#VALUE!
212.3835427

4500000
4000000
3500000
3000000
2500000
Peak Area
2000000
1500000
1000000
500000

Summation
SD
80.87026217 6.358862

Calibration Curve: Caffeine


4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
0

y = 14008x + 494513
R = 0.9969

Series1
Linear (Series1)

100

200

Concentration (ppm)

300

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