If your autoload function is a class method, you can call spl_autoload_register with an array specifying the class and the method to run.

* You can use a static method :
<?php

class MyClass {
  public static function autoload($className) {
    // ...
  }
}

spl_autoload_register(array('MyClass', 'autoload'));
?>

* Or you can use an instance :
<?php
class MyClass {
  public function autoload($className) {
    // ...
  }
}

$instance = new MyClass();
spl_autoload_register(array($instance, 'autoload'));
?>