Developing a simplified method to isolate and characterise EVs from probiotic bacteria: in this brief report, Harshal Sawant, Ji Chen Bihl and Alip Borthakur at Marshall University evaluated ultracentrifugation and precipitation, two widely used extracellular vesicle isolation methods, to establish a standardised protocol for extracting EVs from the probiotics Lactobacillus acidophilus (LA), a Gram-positive bacterium, and Escherichia coli Nissle (EcN), a Gram-negative bacterium. Ultracentrifugation yielded approximately 1.5 times more EVs than precipitation for both strains, with EcN producing a higher EV output than LA https://lnkd.in/eNwBhWZs EVs were quantified and characterised using nanoparticle-tracking analysis (NTA) and Western blotting for surface biomarkers. They indicated that their study's primary limitation was its focus on only one Gram-positive and one Gram-negative probiotic strain, restricting broader comparisons of EV yield, size, and biomarker expression. #extracellularvesicles #bacteria #OMVs #probiotic #Vesiculab
