Cora Vacher’s Post

Check out our recent paper about the effects of aerobic sludge digestion on the quantity of carbapenem resistance genes: https://lnkd.in/g4Vk2b9G We demonstrated the trends, analyzed kinetics and explained the implications to the design of flow-through reactors. We also developed regression models to estimate the quantity of the gene from common design and operation parameters as well as the quantity of other genes.

We tend to talk about heterochromatin quite broadly, but this new paper from Sigurd Braun and colleagues at Justus Liebig University Giessen elegantly dissects the specific heterochromatin regulators acting at distinct constitutive heterochromatin domains of fission yeast. Using more quantitative methods than have been used previously and a systematic approach, they performed genetic screens with reporter genes to identify which factors are required for silencing at specific domains (pericentromeres, subtelomeres, and the silent mating type locus). Out of the 180 mutants that caused a loss of silencing, only 10 were generally required across all heterochromatin domains, whereas the vast majority (108) acted domain specifically. Of these, interestingly, many were involved in metabolic pathways. They also identified a new, critical function in facultative heterochromatin silencing for the uncharacterised protein, Dhm2 (deleterious haploid meiosis), that may be caused by its role in DNA replication or repair. One of our most experienced former journal editors, Sabbi Lall (former editor at NSMB and EIC of Cell Reports) performed a scientific edit of this paper. You can read the full paper using the link below 👇 If you think we might be able to help you with your manuscript, check out our website for more details: www.lifescienceeditors.com https://lnkd.in/etiQEabU

chatomics! Scanpy and Seurat marker gene log2Fold change has a big discrepancy! Do you understand log2Fold change in single-cell RNAseq data? https://lnkd.in/ePiUKxF4 I hope you've found this post helpful. Follow me for more. Subscribe to my FREE newsletter https://lnkd.in/erw83Svn

PASSIGE (Primary Assisted Site-Specific Integrase Genome E) This post, somewhat dense, has been divided into the following chapters, which are developed in the comments. The paper attached to chapter III is, ultimately, the one that originates this post, and you can skip reading the previous chapters (they do not introduce anything new) if you are familiar with these processes and technologies. I.- Introduction to genomic recombination processes II.- General description of PASSIGE III.-Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing

New England Biolabs scientists worked with SLC Management to develop RIM-seq2 to identify methylated loci. This high-throughput technique simultaneously sequences genomes and overlays methylation information, with only minor modifications to existing protocol. RIM-seq2 is compatible with human cells unlike their previously developed RIM-seq, which identified methylase specificity in bacteria. Read more about RIM-seq2 in Genome Research: https://lnkd.in/ecr-t4ea

Another great paper — check it out to read more about RIM-seq2, a strategy that can be used for large-scale methylation identification and simultaneous germline variation, with or without amplification!

🌟Global Nucleic Acid Labelling Market Global Nucleic Acid Labelling Market Size, Trends and Growth Opportunity, By Products (Reagent & Kits and Services), By Labelling Technique (PCR, Nick Translation, Random Primer, In Vitro Transcription, Reverse Transcription, and End Labelling), By Application (DNA Sequencing, PCR, FISH, Microarray, In Situ Hybridization, Blotting, and Other Application), By Region and Forecast Till 2030. By: Analytical Market Research 🔗https://lnkd.in/gSZrN6aR Key Highlight: Market Overview: A detailed overview, market landscape, including market size, growth trends, and key players. Market Segmentation: Analysis of the market by product type, deployment mode, organization size, and industry verticals to identify lucrative opportunities. 📢 Drive into the past to innovate for the future? let’s connect and explore the immersive world of Global Nucleic Acid Labelling Market

This self-paced 'PubChem Training Course' offered by the National Library of Medicine (NIH) has changed my perspective on utilizing PubChem properly. A lot of confusion was cleared regarding PubChem utilization. Here, I have noted the learning outcomes of this course: • Locating information for a specific chemical in PubChem using an accepted identifier or structure. • Searching PubChem with a gene, protein, pathway, or taxonomy to find related chemical information. • Identifying the data source for chemical information in PubChem. • Finding links within PubChem to literature, clinical trials, and reference materials. • Downloading data from PubChem. #PubChem #Nationallibraryofmedicine #nml #nih

An important aspect of vector manufacturing involves minimizing the presence of empty and partial capsids. These capsids, lacking the intended transgene, require higher doses and may potentially trigger heightened immune reactions. Given the potential downstream effects, identifying and characterizing these impurities is critical. Form Bio offers a fully customized characterization report that delivers invaluable insights, providing clarity to your vector designs through long-read sequencing data. Discover more and preview a sample report: https://hubs.ly/Q02tFBc90

Read the article on nucleic acid electrophoresis troubleshooting: https://lnkd.in/gNakC-sC In this Biocompare article, Oliver Glenn H., Global Product Manager for Gene Expression from Bio-Rad Laboratories, explains how incorrect PCR temperatures and cycling times can lead to unexplained presence of faint bands or absence of bands during nucleic acid electrophoresis, and how PCR instruments with thermal gradient features enable accurate determination of optimal annealing temperatures. Learn more about Bio-Rad’s PTC Tempo Thermal Cyclers ➡️ https://lnkd.in/gB3j_N4w #PCR #Electrophoresis #Genomics