Biocompare article on nucleic acid electrophoresis troubleshooting

Youtube link - https://lnkd.in/g8btYk97 PCR or Polymerase Chain Reaction is on of the most basic, most versatile and indispensable techniques in Biotechnology. It is an elegant example of how the principles of Nucleic acid hybridization and DNA replication can be applied to exponentially amplify a specific region of DNA. A critical factor in the success of PCR amplification is the design of specific primers. In this short video, I describe the basic concepts in Primer designing and then design specific primers to amplify the GAPDH gene in the human genome. The required sequence is retrieved from the UCSC Genome Browser and the primers are designed using Primer3. #Primer #PCR #Polymerase_Chain_Reaction #Primer3 #UCSC_Genome_Browser #GAPDH #Primer_designng #lets_grow_together #vipins_e_classroom #tm

Let's fix the peer-review crisis, improve quality, and set an open, transparent, and ethical standard for peer-review. Publish better science, 10x faster! Sign up now: https://xpeerd.com For the example in this video, check our our open review via Disqus on "Ultra-Efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids" by Alexander Pisera et al, here: https://disq.us/p/31775ry

Researchers report two new genetically encoded tags based on single chain variable fragments (scFv) of human antibodies that non-covalently bind either Cy3 or Cy5 with high specificity and selectivity. These tags, termed PEPCy, additionally enhance brightness, photostability, and allow for wash-free imaging. The authors demonstrate these labels for #LiveCell #SuperResolution single molecule localization #Microscopy #SMLM of surface receptors on both mammalian and bacterial cells (performed using a Nikon Ti2 microscope). Click to view the preprint article: https://bit.ly/3xX3xcj

PASSIGE (Primary Assisted Site-Specific Integrase Genome E) This post, somewhat dense, has been divided into the following chapters, which are developed in the comments. The paper attached to chapter III is, ultimately, the one that originates this post, and you can skip reading the previous chapters (they do not introduce anything new) if you are familiar with these processes and technologies. I.- Introduction to genomic recombination processes II.- General description of PASSIGE III.-Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing

Faster, smaller, smarter: intelligent manufacturing of #mRNA through the power of process analytical technologies The #RNAtherapeutics field is rapidly expanding, with applications from #cancervaccines to gene editing. To avoid manufacturing bottlenecks that slow innovation, it is crucial to adopt technologies that allow developers to measure and control production processes in real time, ensuring final product quality and accelerated timelines. Next month, ReciBioPharm’s Aaron Cowley (CSO) and Edita Botonjic-Sehic (Senior Director, Process Sciences) will discuss the benefits of integrating #processanalyticaltechnologies (PAT) within #mRNAmanufacture and explore the platform’s ability to predict and monitor #CQAs, including #RNA concentration and #nucleosidetriphosphate consumption. Register for this upcoming #webinar below:

Find your target. Our functional genomic tools enable comprehensive screening of potential targets, while our cell culture tools help you validate using the most predictive model for your project. Explore more on our mAb target discovery pages. #mAbs

Now You Know About TARGATT™ ✨ TARGATT™ technology enables single copy integration of your large gene of interest at an "attP" landing pad in a preselected safe harbor locus. We've harnessed this technology to engineer TARGATT™ "master" HEK293 and CHO cell lines for our ready-to-use knock-in kits. These user-friendly kits allow you to perform GOI knock-ins in your own lab! The TARGATT™ cell lines have demonstrated high gene knock-in efficiency levels and offer several advantages over traditional genome editing methods, such as unidirectional site-specific integration and the ability to support library construction. Is this the right genome editing technology for you? Send us your questions @ [email protected] or learn more @ https://lnkd.in/gbDzcDC3 & https://lnkd.in/geZD9A5V #geneediting #celltherapy #AppliedStemCell #TARGATT

🎥 This video demonstrates the identification of HUB genes from a complex gene network. By watching this video, you'll learn how to analyze a microarray dataset using GEO2R analysis and identify differentially expressed genes (DEGs). Additionally, you'll be able to use the DEGs to build a complex interactive network and further analyze it to identify your HUB genes using Cytoscape software. 🔬🧬 #bioinformatics #molecularbiology Click on the link below; https://lnkd.in/dj5nSr-A

🧬 Having troubles with your cloning experiments? --> Have you tried the Gibson Assembly technique? With Gibson Assembly, you can easily build complex plasmids with no need of common restriction sites. BONUS: you can edit your sequences at the single base scale, absolute requirement for CRISPR/Cas9 mutagenesis! Here is my 100% success protocol for Gibson Assembly; I never failed any cloning with it, even for complex sequences! Through this practical case, you will learn how to assemble two genes with a linker inside a plasmid; typical case when you want to fuse a GFP in 3' of a gene! #Cloning #GibsonAssembly #CRISPR

Faster, smaller, smarter: intelligent manufacturing of #mRNA through the power of process analytical technologies The #RNAtherapeutics field is rapidly expanding, with applications from #cancervaccines to gene editing. To avoid manufacturing bottlenecks that slow innovation, it is crucial to adopt technologies that allow developers to measure and control production processes in real time, ensuring final product quality and accelerated timelines. This month, ReciBioPharm’s Aaron Cowley (CSO) and Edita Botonjic-Sehic (Senior Director, Process Sciences) will discuss the benefits of integrating #processanalyticaltechnologies (PAT) within #mRNAmanufacture and explore the platform’s ability to predict and monitor #CQAs, including #RNA concentration and #nucleosidetriphosphate consumption. Register for this upcoming #webinar below: