Jonathon Foos

Happy Thursday all! Check out this open access Nature Leukemia review by Lei et al., "Leveraging CRISPR gene editing technology to optimize the efficacy, safety and accessibility of CAR T-cell therapy." https://lnkd.in/gAWFHq-j. Abstract: Chimeric Antigen Receptor (CAR)-T-cell therapy has revolutionized cancer immune therapy. However, challenges remain including increasing efficacy, reducing adverse events and increasing accessibility. Use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology can effectively perform various functions such as precise integration, multi-gene editing, and genome-wide functional regulation. Additionally, CRISPR screening using large-scale guide RNA (gRNA) genetic perturbation provides an unbiased approach to understanding mechanisms underlying anti-cancer efficacy of CAR T-cells. Several emerging CRISPR tools with high specificity, controllability and efficiency are useful to modify CAR T-cells and identify new targets. In this review we summarize potential uses of the CRISPR system to improve results of CAR T-cells therapy including optimizing efficacy and safety and, developing universal CAR T-cells. We discuss challenges facing CRISPR gene editing and propose solutions highlighting future research directions in CAR T-cell therapy. #drugdiscovery #cancerresearch #crispr #tcelltherapy #immunooncology #immunotherapy #scientificresearch

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Happy Friday all! Check out this open access Nature Scientific Reports article by Abali et al., "A nanowell platform to identify, sort and expand high antibody-producing cells." https://lnkd.in/gMuJNSxf Abstract: Increased use of therapeutic monoclonal antibodies and the relatively high manufacturing costs fuel the need for more efficient production methods. Here we introduce a novel, fast, robust, and safe isolation platform for screening and isolating antibody-producing cell lines using a nanowell chip and an innovative single-cell isolation method. An anti-Her2 antibody producing CHO cell pool was used as a model. The platform; (1) Assures the single-cell origin of the production clone, (2) Detects the antibody production of individual cells and (3) Isolates and expands the individual cells based on their antibody production. Using the nanowell platform we demonstrated an 1.8–4.5 increase in anti-Her2 production by CHO cells that were screened and isolated with the nanowell platform compared to CHO cells that were not screened. This increase was also shown in Fed-Batch cultures where selected high production clones showed titers of 19–100 mg/L on harvest day, while the low producer cells did not show any detectable anti-Her2 IgG production. The screening of thousands of single cells is performed under sterile conditions and the individual cells were cultured in buffers and reagents without animal components. The time required from seeding a single cell and measuring the antibody production to fully expanded clones with increased Her-2 production was 4–6 weeks. #drugdiscovery #antibodies #cancerresearch #immunotherapy #immunooncology #scientificresearch

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Happy Tuesday all! Check out this open access Immunological Review by Yu et al., "Single domain antibody: Development and application in biotechnology and biopharma." Summary: Heavy-chain antibodies (HCAbs) are a unique type of antibodies devoid of light chains, and comprised of two heavy chains-only that recognize their cognate antigen by virtue of a single variable domain also referred to as VHH, single domain antibody (sdAb), or nanobody (Nb). These functional HCAbs, serendipitous discovered about three decades ago, are exclusively found in camelids, comprising dromedaries, camels, llamas, and vicugnas. Nanobodies have become an essential tool in biomedical research and medicine, both in diagnostics and therapeutics due to their beneficial properties: small size, high stability, strong antigen-binding affinity, low immunogenicity, low production cost, and straightforward engineering into more potent affinity reagents. The occurrence of HCAbs in camelids remains intriguing. It is believed to be an evolutionary adaptation, equipping camelids with a robust adaptive immune defense suitable to respond to the pressure from a pathogenic invasion necessitating a more profound antigen recognition and neutralization. This evolutionary innovation led to a simplified HCAb structure, possibly supported by genetic mutations and drift, allowing adaptive mutation and diversification in the heavy chain variable gene and constant gene regions. Beyond understanding their origins, the application of nanobodies has significantly advanced over the past 30 years. Alongside expanding laboratory research, there has been a rapid increase in patent application for nanobodies. The introduction of commercial nanobody drugs such as Cablivi, Nanozora, Envafolimab, and Carvykti has boosted confidence among in their potential. This review explores the evolutionary history of HCAbs, their ontogeny, and applications in biotechnology and pharmaceuticals, focusing on approved and ongoing medical research pipelines. #drugdiscovery #nanobodies #cancerresearch #therapeutics #immunology #antibodies #scientificresearch

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📍 Harnessing single-cell and spatial transcriptomics for crop improvement Hu et al. presented an update on the application of single-cell and spatial transcriptomics technologies in crop improvement. Key points 🌱 Insights into the cell-specific transcriptomic responses of plants to various environmental stimuli 🌱 The discovery of novel genes and gene regulatory networks for genetic manipulation and breeding strategies aimed at enhancing crop yield, quality, and resilience. ➡️ More details: https://lnkd.in/eEPSx6Ya #spatialomics #spatialbiology #singlecellanalysis #singlecell

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Using an innovative reporter line, this study sheds light on the complex interplay between genome editing components, vector choices, and cellular stress responses, offering insights for optimizing gene therapy protocols.  https://lnkd.in/eQBFusZr

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Please join Abiosciences on Sept 19th at 10x Genomics User Group meeting at Grand Hyatt - SFO (9:30 AM - 3:00 PM PT). Topics include: 1. Chromium GEM-X Single Cell Assays 2. Visium HD 3. Xenium Multimodal Segmentation, 5K, and In-line Protein 4. Data analysis Stop by our table to speak to our experts to discuss scRNA, spatial transcriptomics or data-analysis.

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Happy Wednesday all! Check out this open access Cell Immunity review by Jason G. Cyster and Patrick C. Wilson, "Antibody modulation of B cell responses—Incorporating positive and negative feedback." Summary: Antibodies are powerful modulators of ongoing and future B cell responses. While the concept of antibody feedback has been appreciated for over a century, the topic has seen a surge in interest due to the evidence that the broadening of antibody responses to SARS-CoV-2 after a third mRNA vaccination is a consequence of antibody feedback. Moreover, the discovery that slow antigen delivery can lead to more robust humoral immunity has put a spotlight on the capacity for early antibodies to augment B cell responses. Here, we review the mechanisms whereby antibody feedback shapes B cell responses, integrating findings in humans and in mouse models. We consider the major influence of epitope masking and the diverse actions of complement and Fc receptors and provide a framework for conceptualizing the ways antigen-specific antibodies may influence B cell responses to any form of antigen, in conditions as diverse as infectious disease, autoimmunity, and cancer. #drugdiscovery #cancerresearch #antibodies #infectiousdisease #sarscov2 #autoimmunity #immunooncology #immunotherapy #scientificresearch

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#Bioanalytical #drugdevelopment can be challenging. Come join us for a day of learning and sharing your concerns (or experiences) at PBSS International workshop "Current State of Bioanalysis for Biologics and Cell/Gene Therapy Products" Refining Established Approaches for New Complex Therapies" #cellandgenetherapies #biologics

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New publication alert! Check out our latest article "Complex In Vitro Model Characterization for Context of Use in Toxicologic Pathology: Use Cases by Collaborative Teams of Biologists, Bioengineers, and Pathologists" at the link below: https://lnkd.in/gAc9ADhC

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Happy Monday! Make sure to check out one of our most recent publications: "Complex in Vitro Model Characterization for Context of Use in Toxicologic Pathology: Use Cases by Collaborative Teams of Biologists, Bioengineers, and Pathologists"

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Parse Biosciences Partners with OMAPiX to Expand Access to Single Cell Sequencing https://lnkd.in/g-JD-ubt SEATTLE–(BUSINESS WIRE)–Parse Biosciences Partners with OMAPiX to Expand Access to Single Cell Sequencing Click here to view original post Click Here to Publish/Feature Your Company or Product News with Biotech Networks

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Our Technical Genomics team has extensive experience working and innovating with #longread sequencing. In this article, discover how they're working with researchers to generate long and ultra-long reads. https://okt.to/wNZVfU #genomics #sequencing #longreads #DNAreplication

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It’s out! Check out Monserrat’s article entitled "Navigating the Signaling Network: RTK and GPCR Crosstalk Uncovered" in the Dr. GPCR Ecosystem today! Become a site member and enjoy more GPCR content! ➡️https://bit.ly/3yZaxpt #gpcr #drgpcr

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Interested in the discovery and applications of aptamers in biosensing and cell separations? If so, don't miss this Review article "Aptamers 101: Discovery & Applications in Biosensors and Separations" In this work, Suzie H. Pun et al. describe the major steps of the library selection process for aptamers, evaluate recently developed aptamer biosensors for SARS-CoV-2 virus detection and discuss aptamer-based separations for partitioning different molecules or cell types. https://lnkd.in/eagb4dSf

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Toxicogenomics has several applications including precision toxicology, biomarker discovery, human health assessment, environmental toxicology, and pharmaceutical safety. Learn more about how NGS transforms the toxicogenomics field in our blog. https://lnkd.in/dzAWytin #genetic #toxicology #ngs #biomarkers #pharma

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Happy Tuesday all! Check out this open access Cancer Cell paper by Seed et al., "Elucidating acquired PARP inhibitor resistance in advanced prostate cancer." https://lnkd.in/gJSkh8dJ Highlights: • Monitoring of PARPi-treated mCRPC detects the emergence of resistant subclones. • By 16 weeks, these associate with disease progression and survival. • BRCA2/PALB2 reversions in mCRPC present signs of microhomology-mediated repair. • Tumors with BRCA2 HomDels resist PARPi through the selection of BRCA2-competent cells. Looking for your PARPi small molecule breakthrough? Check out our unparalleled PARP & PARPTrap™ portfolio of biochemical and cellular assay kits and screening services to facilitate your early stage drug discovery pipeline: https://lnkd.in/g9kJrq6e #drugdiscovery #cancerresearch #parp #brca #immunooncology #scientificresearch

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