Carina Clingman, PhD

Carina Clingman, PhD

Greater Boston
17K followers 500+ connections

About

𝑷𝒉𝑫 𝑺𝒄𝒊𝒆𝒏𝒕𝒊𝒔𝒕 𝑻𝒖𝒓𝒏𝒆𝒅 𝑭𝒓𝒂𝒄𝒕𝒊𝒐𝒏𝒂𝒍 𝑯𝒆𝒂𝒅 𝒐𝒇 𝑻𝑨 👉Helping…

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Experience

  • Recruitomics Biotalent Consulting Graphic
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    United States

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    Manchester, New Hampshire, United States

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    Greater Boston Area

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    Worcester, MA

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    San Luis Obispo, California Area

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    Middleburg, VA

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    San Luis Obispo, California Area

Education

  • UMass Chan Medical School Graphic

    University of Massachusetts Medical School

    Activities and Societies: GSBS Cares community service chair 2010-2012

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    Activities and Societies: Undergraduate researcher - Dr. Dan Peterson, MAREC equine nutrition intern - Dr. Burt Staniar, Horse breeding enterprise - Dr. Matt Burd

    GPA: 3.95

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    Activities and Societies: 'A Stable Place' Therapeutic Horseback Riding Instructor

Volunteer Experience

  • Chairman

    GSBS Cares

    - 2 years

    Education

    We organized roughly one volunteer activity per month. Activities ranged from mentoring elementary school children to building homes to participating in medical awareness events.

  • Big Brothers Big Sisters Graphic

    Big Sister

    Big Brothers Big Sisters

    - 2 years 1 month

    Children

    Tutored, advised, and generally had fun with a little sister in the program.

Publications

  • A Conserved Three-Nucleotide Core Motif Defines Musashi RNA-Binding Specificity

    Journal of Biological Chemistry

    Musashi (MSI) family proteins control cell proliferation and differentiation in many biological systems. They are over-expressed in tumors of several origins, and their expression level correlates with poor prognosis. MSI proteins control gene expression by binding RNA and regulating its translation. They contain two RNA recognition motif (RRM) domains, which recognize a defined sequence element. The relative contribution of each nucleotide to the binding affinity and specificity is unknown. We…

    Musashi (MSI) family proteins control cell proliferation and differentiation in many biological systems. They are over-expressed in tumors of several origins, and their expression level correlates with poor prognosis. MSI proteins control gene expression by binding RNA and regulating its translation. They contain two RNA recognition motif (RRM) domains, which recognize a defined sequence element. The relative contribution of each nucleotide to the binding affinity and specificity is unknown. We analyzed the binding specificity of three MSI family RRM domains using a quantitative fluorescence anisotropy assay. We found that the core element driving recognition is the sequence UAG. Nucleotides outside of this motif have a limited contribution to binding free energy. For mouse MSI1, recognition is determined by the first of the two RRM domains. The second RRM adds affinity but does not contribute to binding specificity. In contrast, the recognition element for Drosophila Msi is more extensive than the mouse homolog, suggesting functional divergence. The short nature of the binding determinant suggests that protein-RNA affinity alone is insufficient to drive target selection by MSI family proteins.

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  • Metabolite sensing in eukaryotic mRNA biology

    Wires RNA

    All living creatures change their gene expression program in response to nutrient availability and metabolic demands. Nutrients and metabolites can directly control transcription and activate second-messenger systems. More recent studies reveal that metabolites also affect post-transcriptional regulatory mechanisms. Here, we review the increasing number of connections between metabolism and post-transcriptional regulation in eukaryotic organisms. First, we present evidence that riboswitches, a…

    All living creatures change their gene expression program in response to nutrient availability and metabolic demands. Nutrients and metabolites can directly control transcription and activate second-messenger systems. More recent studies reveal that metabolites also affect post-transcriptional regulatory mechanisms. Here, we review the increasing number of connections between metabolism and post-transcriptional regulation in eukaryotic organisms. First, we present evidence that riboswitches, a common mechanism of metabolite sensing in bacteria, also function in eukaryotes. Next, we review an example of a double stranded RNA modifying enzyme that directly interacts with a metabolite, suggesting a link between RNA editing and metabolic state. Finally, we discuss work that shows some metabolic enzymes bind directly to RNA to affect mRNA stability or translation efficiency. These examples were discovered through gene-specific genetic, biochemical, and structural studies. A directed systems level approach will be necessary to determine whether they are anomalies of evolution or pioneer discoveries in what may be a broadly connected network of metabolism and post-transcriptional regulation. WIREs RNA 2013, 4:387-396. doi: 10.1002/wrna.1167 Conflict of interest: The authors have declared no conflicts of interest for this article. For further resources related to this article, please visit the WIREs website.
    Copyright © 2013 John Wiley & Sons, Ltd.

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  • Quaking regulates Hnrnpa1 expression through its 3' UTR in oligodendrocyte precursor cells

    PLoS Genetics

    In mice, Quaking (Qk) is required for myelin formation; in humans, it has been associated with psychiatric disease. QK regulates the stability, subcellular localization, and alternative splicing of several myelin-related transcripts, yet little is known about how QK governs these activities. Here, we show that QK enhances Hnrnpa1 mRNA stability by binding a conserved 3' UTR sequence with high affinity and specificity. A single nucleotide mutation in the binding site eliminates QK-dependent…

    In mice, Quaking (Qk) is required for myelin formation; in humans, it has been associated with psychiatric disease. QK regulates the stability, subcellular localization, and alternative splicing of several myelin-related transcripts, yet little is known about how QK governs these activities. Here, we show that QK enhances Hnrnpa1 mRNA stability by binding a conserved 3' UTR sequence with high affinity and specificity. A single nucleotide mutation in the binding site eliminates QK-dependent regulation, as does reduction of QK by RNAi. Analysis of exon expression across the transcriptome reveals that QK and hnRNP A1 regulate an overlapping subset of transcripts. Thus, a simple interpretation is that QK regulates a large set of oligodendrocyte precursor genes indirectly by increasing the intracellular concentration of hnRNP A1. Together, the data show that hnRNP A1 is an important QK target that contributes to its control of myelin gene expression.

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  • Quantitative approaches to monitor protein-nucleic acid interactions using fluorescent probes

    RNA

    Sequence-specific recognition of nucleic acids by proteins is required for nearly every aspect of gene expression. Quantitative binding experiments are a useful tool to measure the ability of a protein to distinguish between multiple sequences. Here, we describe the use of fluorophore-labeled oligonucleotide probes to quantitatively monitor protein/nucleic acid interactions. We review two complementary experimental methods, fluorescence polarization and fluorescence electrophoretic mobility…

    Sequence-specific recognition of nucleic acids by proteins is required for nearly every aspect of gene expression. Quantitative binding experiments are a useful tool to measure the ability of a protein to distinguish between multiple sequences. Here, we describe the use of fluorophore-labeled oligonucleotide probes to quantitatively monitor protein/nucleic acid interactions. We review two complementary experimental methods, fluorescence polarization and fluorescence electrophoretic mobility shift assays, that enable the quantitative measurement of binding affinity. We also present two strategies for post-synthetic end-labeling of DNA or RNA oligonucleotides with fluorescent dyes. The approaches discussed here are efficient and sensitive, providing a safe and accessible alternative to the more commonly used radio-isotopic methods.

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Courses

  • Chemical Biology

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  • GSBS Core

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  • Neurodevelopment

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  • Python Programming

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  • RNA Biology

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Projects

  • Graduate Student Recruitment

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    Traveled to various universities to present our graduate school opportunities to undergraduate students.

    Other creators

Honors & Awards

  • Graduate School of Biomedical Sciences Honor Board

    University of Massachusetts Medical School

    Chosen to investigate and uphold matters of scholarly conduct within the graduate school

  • Community Service Award

    University of Massachusetts Medical School

    Award for outstanding community service activities

  • Graduate Student Body Committee Representative

    University of Massachusetts Medical School

  • Academic Excellence Award

    California Polytechnic State University Animal Science Department

    Highest cumulative GPA in graduating class

  • Undergraduate Academic Award

    Journal of Animal Science

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