Andrey Gorin

As a result of in vivo interactions, a protein corona layer is formed around extracellular vesicles. In their review, MORTEZA HEIDARZADEH, Amir Zarebkohan, Reza Rahbarghazi, and Emel Sokullu investigate different aspects of such layer (review article available at: https://lnkd.in/dnHvGaj3). One point, though, raises a question in minds, which regards the removal and/or alteration of EV corona by size exclusion chromatography (SEC), one of the most widely used EV purification method. This also brings to minds the concerns about the standardization of the isolation tool for downstream applications. Hence, here is our new question for our EV Lab Quiz: What are the implications of the alteration of EV corona by SEC? Do not hesitate to drop your comments! #extracellularvesicles #exosomes #EVcorona #sizeexclusionchromatography

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📢 Cereno Scientific will present preclinical data for drug candidate CS585 at the American Society of Hematology Annual Meeting and Exposition 2024, San Diego, 7-10 December 2024.   Abstract title: Selectivity and Long Action of In Vivo Efficacy of CS585, a Novel Prostacyclin Receptor Agonist, Compared to FDA-Approved Prostacyclin Agonists Iloprost and Selexipag in the Prevention of Thrombosis Authors: L. Stanger, M Flores, K Goerger and M. Holinstat, at the University of Michigan, Ann Arbor, USA; and B. Dahlöf, Cereno Scientific.   The poster will be presented by Livia Stanger, Graduate student at the Holinstat Lab at University of Michigan, led by Prof. Michael Holinstat, Professor in the Department of Pharmacology, the Department of Internal Medicine (Division of Cardiovascular Medicine), and the Department of Vascular Surgery at the University of Michigan. Prof. Holinstat leads Cereno’s development programs at the University of Michigan and is Director of Translational Research at Cereno.   📍 Presentation Date: Sunday, 8 December 2024, 18:00–20:00 PST 👉 Read more about ASH Annual Meeting and Exposition 2024: https://lnkd.in/dYZtUjZ #drugdevelopment #thrombosis #hematology

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🌟 Speaker Spotlight: Gülçin Arslan Azizoğlu, PhD 🌟 We're excited to announce Gülçin Arslan Azizoğlu, Research Scientist II and Associate Director of the Laboratory of Drug Delivery at Georgia Institute of Technology, as a featured speaker at the Microneedle & Intradermal Delivery Forum 2024. Despite advancements in contraceptive methods, the need for effective and accessible options remains critical worldwide. Dr. Azizoğlu's research focuses on advancing contraceptive technology through microneedle (MN) patches designed for long-term use, self-administration, and global applicability. Her presentation will highlight ongoing efforts to develop a six-month contraceptive MN patch, covering key aspects such as formulation, dose delivery, skin penetration, sterilization, and patch stability. These innovations aim to enhance patient compliance and expand contraceptive choices in clinical and public health settings. Don't miss this opportunity to gain insights into the future of contraceptive technology from a leader in drug delivery research. Join us at the Microneedle & Intradermal Delivery Forum 2024! Learn more here: https://lnkd.in/ei2GPXMt #DrugDelivery #Contraception #MicroneedlePatches #GlobalHealth #DrugDevices

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A closed lecture by ILYA FOMINTSEV on cancer and the problem of oncology was held in Chisinau. We discussed important topics and answered burning questions with the participants: - What is cancer? How to reduce the likelihood of dying from it: scientifically based methods and paradoxes of cancer prevention. - What is evidence-based medicine? How does post-Soviet medicine differ from world medicine? - How is modern Western medical education structured? How does it differ from education in the post-Soviet space? You can watch an excerpt from the lecture here: https://lnkd.in/ecKvV4b5.

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Israeli-led consortium publishes cell atlas of the human body: The first draft of the Human Cell Atlas, a comprehensive database of human cells, has been published. The project, launched in 2016, aims to create comprehensive reference maps of all human cells as a basis for understanding human health and for diagnosing, monitoring, and treating disease. https://lnkd.in/gD4idDbZ

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🆕New in #JEV 🆕 A novel multiplexed immunoassay for surface-exposed proteins in plasma extracellular vesicles Emma Tordoff, Jillian Allen, Katya Elgart, Ahmed Elsherbini, Vrinda Kalia, Haotian Wu, Erden Eren, Dimitrios Kapogiannis, Olesia Gololobova, Kenneth Witwer, Olga Volpert, Erez Eitan Small membranous extracellular vesicles (EV) incorporate proteins and nucleic acids from the parent cell. Proteins exposed on EV surface are dictated by cellular origin and biogenesis pathway. To better understand the EV origin and function, it is important to develop methods that reveal surface protein composition of heterogeneous EV populations in culture supernatants and in biofluids. Tetraspanins CD9, CD63, and CD81 are common and abundant EV markers. However, their relative enrichment (profile) on EVs of specific cellular origins is not fully elucidated. We introduce LuminEV, a novel version of the Luminex assay for the multiplexed analysis of EV surface proteins. Optimized LuminEV reagents enable direct, specific, and sensitive measurements of EV markers in biofluids and in culture supernatants, bypassing EV isolation step. LuminEV assay for CD9, CD63, and CD81 was validated by comparing simplex and multiplex measurements, establishing linearity, spike-in recovery, inter- and intra-assay precision, and reproducibility between operators. LuminEV measurements of CD9, CD63, and CD81 in conditioned media from 15 cell lines revealed strong variations between cell types and showed high sensitivity, which enabled EV detection without prior concentration. Using tetraspanin levels as a readout, we noted suppression and induction of EV release from the cultured cells by GW6869 and monensin. Measurement of EV CD9, CD63, and CD81 in blood plasma from 70 disease-free donors showed respective abundance of 72, 16, and 12%. CD63 displayed weak, albeit significant, negative correlation with age and was slightly lower in female samples. The assay was then used to detect cell type-specific EV surface markers, including CD235a (erythrocytes), GAP43 (neurons), and CD68 (macrophages), and to detect differences in tetraspanin profiles between healthy and diseased donors. In summary, LuminEV offers robust and sensitive approach for multiplexed assessment of EV surface proteins, to facilitate the research into EV biology, biomarker, and therapeutic applications. https://lnkd.in/eUsJWDZQ

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As Can Sucak Lab, we published the largest PID cohort ever studied in Turkiye. The study includes more than 300 patients, of those 57 novel, 127 disease-causing variants and more. However, perhaps more important than the content of the study itself is that this study demonstrates that Can Sucak's legacy is being preserved, reflects his contribution to patients, and proves the collaboration of Turkish primary immunodeficiency community.  Read here: https://lnkd.in/dpx2V8Nc Baran Erman/Can Sucak Lab Journal of Clinical Immunology

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Iranian Journal of Allergy, Asthma and Immunology Vol 23 No 6 (2024) https://ijaai.tums.ac.ir/ Table of Contents 1. Molecular Pathways Underlying the Therapeutic Effect of Stem Cells during Asthmatic Changes (Review Article) 2. The Role of Th17/Treg Imbalance, FeNO, Eosinophils, IgE and Their Correlation with Lung Function Parameters with Asthma-chronic Obstructive Pulmonary Disease 3. Clinical Analysis of Modified Extended Endoscopic Sinus Surgery for Chronic Rhinosinusitis with Nasal Polyps and Allergic Rhinitis 4. Exploring the Association between Blood Indices and Skin and Joint Activity of Psoriatic Arthritis 5. Increased Cytotoxic CD4+ T Cells with Reduced Cytotoxic Gene Profile Expression in Cytomegalovirus Reactivated Kidney Transplant Patients 6. Effect of Exercise, MitoQ, and Their Combination on Inflammatory and Gene Expression in Women with Multiple Sclerosis 7. Ameliorative Effect of Melittin Encoded DNA Plasmid in an Ovalbumin-induced Murine Model of Allergy 8. A Novel Prognostic Immune-related Gene Signature in Hepatocellular Carcinoma Through Bioinformatics and Experimental Approaches 9. MicroRNA-486-3p Targets Chymotrypsin C to Regulate Pancreatic Cancer Progression and Immunosuppressive Factor Expression 10. Bioinformatics-driven Identification of lncRNA LINC02381 in Mediating Cisplatin Resistance via IL-12 Induced Wnt/TCF7 Signaling in Ovarian Cancer 11. How Hospitalizations Can Be Effective in Subsequent Care of Children with Asthma? (Brief Communication) 12. Association of Toxoplasmosis with Serum TGF-β, IL-17, and IL-6 Levels in Individuals with Diabetes (Brief Communication)

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📢 Just published in the November issue 🆕 The latest research highlights the antitumor effects of Scleromitrion diffusum (Willd.) R. J. Wang in gastric cancer (GC). 🧬 Published in Turkish Journal of Gastroenterology, the study reveals how this herb inhibits GC growth and metastasis by targeting the ERBB2/ERBB3/PI3K/AKT pathway. This multi-target approach shows promising potential for GC treatment. 📊 👉🏼 https://lnkd.in/dyMQy8T8 #GastricCancer #CancerResearch #ScleromitrionDiffusum #NovemberIssue #Published #Gastroenterology #PI3KPathway #Antitumor

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"🔬 Thrilled to announce our latest publication! Delve into the intricate world of Leishmania donovani with our research on 'Anhydroparthenin as a dual-target inhibitor against Sterol C-24 methyltransferase and Sterol 14-α demethylase.' This comprehensive study, combining in vitro and in silico analyses, sheds light on promising avenues for combating this disease. Read the full article here: https://lnkd.in/dBs3p8pf #Research #Leishmania #Science"

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On this day in science Ferid Murat was born. Murat was a American physician and pharmacologist who was a co-winner of the 1998 Nobel Prize for Physiology or Medicine for discovering that a gas, nitric oxide, acts as a signalling molecule in the cardiovascular system. This work, performed in the 1980's, uncovered an entirely new mechanism for how blood vessels in the body relax and widen. It led to the development of the anti-impotence drug Viagra and potential new approaches for understanding and treating other diseases. He was a co-worker with Robert F. Furchgott and Louis J. Ignarro. #chemistry #chemical #structural #inorganicchemistry #inorganic #science #scienceandtechnology #sciencecommunication #scienceeducation #nitricoxide #cardiovascular #nitrosamines #nitrosamine

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Updates on the GI and Neuroendocrine Tumors working group https://lnkd.in/eKReaFtr Immune Oncology Research Institute Yeolyan Hematology and Oncology Center, Ministry of Health of Armenia Lilit Harutyunyan Philip Agop Philip #Cancer #IMMONC #GI #NeuroEndocrine #GastroIntestinal #Collaboration #CancerResearch #Medicine #Health #Oncology #OncoDaily

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A new study recently published in Genome Medicine has unveiled significant genetic connections between Inflammatory Bowel Disease (IBD) and Parkinson’s Disease (PD). The research team, led by Meltem Ece Kars, Yuval Itan, and Inga Peter, employed advanced genomic analysis techniques to investigate rare genetic variants in patients diagnosed with both conditions. Their discovery of shared mutations in the #LRRK2 gene sets a new precedent for integrated treatment strategies. This research not only deepens our understanding of the genetic underpinnings common to these chronic diseases but also pioneers the development of dual-disease therapies. Such innovative approaches are poised to transform patient care by targeting both neurological and gastrointestinal symptoms with a single, cohesive treatment plan. "By pinpointing the genetic underpinnings common to both IBD and PD, we pave the way for innovative treatments, whether through the development of novel drug targets or the repurposing of existing drugs, that could potentially tackle the root causes of these conditions," Dr. Kars said. Learn more: https://lnkd.in/ed-d9yyD #IBD #Parkinsons #ParkinsonsDisease #InflammatoryBowelDisease Icahn School of Medicine at Mount Sinai Mount Sinai Health System The Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai

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𝗕𝗲𝘀𝘁 𝗣𝗵𝗗 𝗧𝗵𝗲𝘀𝗶𝘀 𝗔𝘄𝗮𝗿𝗱 𝗴𝗿𝗮𝗻𝘁𝗲𝗱 𝘁𝗼 𝗩𝗲𝗿𝗮 𝗜𝘀𝗰𝗮 Vera Isca has been honored with the Pharmaceuticals 2024 Best PhD Thesis Award for her work titled "New Class of Protein Kinase C Modulators: From Ethnopharmacological to Nanoparticles." Vera Isca thesis, supervised by Prof. Patrícia Rijo (CBIOS - ULusófona), Prof. Carlos Afonso (iMed - FFUL), and Prof. Lucilia Saraiva (REQUIMTE - LAQV Porto), focused on the extraction, isolation, and derivatization of a lead royleanone from Plectranthus spp. One of the derivatives from this compound has shown exceptional promise in the treatment of breast cancer through Protein Kinase C alpha (PKC-α) activation. https://lnkd.in/dzxA8jDQ #CBIOS #ECTS #ulusofona #BioNatural #NaturalProducts #NaturalProductsChemistry #Pharmacognosy #Pharmacognosy #Phytochemistry #OrganicChemistry #MedicinalChemistry #MDPI #Pharmaceuticals

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Varveri, A., Papadopoulou, M., Papadovasilakis, Z. et al. Immunological synapse formation between T regulatory cells and cancer-associated fibroblasts promotes tumour development. Nat Commun 15, 4988 (2024). https://lnkd.in/dZYrmzCd "Abstract Cancer-associated fibroblasts (CAFs) have emerged as a dominant non-hematopoietic cell population in the tumour microenvironment, serving diverse functions in tumour progression. However, the mechanisms via which CAFs influence the anti-tumour immunity remain poorly understood. Here, using multiple tumour models and biopsies from cancer patients, we report that α-SMA+ CAFs can form immunological synapses with Foxp3+ regulatory T cells (Tregs) in tumours. Notably, α-SMA+ CAFs can phagocytose and process tumour antigens and exhibit a tolerogenic phenotype which instructs movement arrest, activation and proliferation in Tregs in an antigen-specific manner. Moreover, α-SMA+ CAFs display double-membrane structures resembling autophagosomes in their cytoplasm. Single-cell transcriptomic data showed an enrichment in autophagy and antigen processing/presentation pathways in α-SMA-expressing CAF clusters. Conditional knockout of Atg5 in α-SMA+ CAFs promoted inflammatory re-programming in CAFs, reduced Treg cell infiltration and attenuated tumour development. Overall, our findings reveal an immunosuppressive mechanism entailing the formation of synapses between α-SMA+ CAFs and Tregs in an autophagy-dependent manner."

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On June 17th, Dr. Jerome H. Kim, Director General of IVI, and Prof. Yazdan Yazdanpanah, Director of the ANRS-Emerging Infectious Diseases Agency (ANRS-MIE), signed a Memorandum of Understanding (MoU) to collaborate in preclinical and clinical research to develop accessible, suitable, and affordable medical interventions to tackle emerging and re-emerging infectious diseases in low—and middle-income countries (LMICs). This initiative will engage collaborators of the ANRS-MIE and IVI international networks, which span countries including Brazil, Burkina Faso, Cambodia, Cameroon, Cote d’Ivoire, the Democratic Republic of the Congo, Guinea, Mali, Senegal, Vietnam, and Zambia. This MoU not only underscores our shared commitment to combating infectious diseases in the hardest-hit regions but also ignites a new era of collaboration and innovation. By joining our expertise, we can significantly strengthen clinical research capacities in LMICs. Together, we are well-positioned to make a profound and lasting impact on global health.

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