Vesiculab

Investigating plasma- and adipose-derived MSC-EVs as potential therapeutic platforms for neuroinflammation: in this recent article, Rummenigge Oliveira Silva, Charles Ramassamy at INRS-Institut Armand-Frappier and collaborators demonstrated that human plasma-derived EVs exhibit greater brain-targeting specificity, while adipose-derived mesenchymal stem cell EVs provide regenerative and immunomodulatory benefits. They further explored the potential of these EVs as therapeutic carriers for brain-targeted drug delivery using Donepezil (DNZ), a cholinesterase inhibitor with neuroprotective and anti-inflammatory properties commonly used for Alzheimer's disease https://lnkd.in/gxnxE_45 In vivo experiments with zebrafish larvae showed that both EV formulations reduced microglial proliferation and exhibited antioxidant effects. Their findings highlight the promise of DNZ-loaded EVs as a novel strategy for treating neuroinflammation associated with neurodegenerative diseases. An article co-authored by Mohamed Haddad, Hermine Counil, Charlotte Zaouter, Shunmoogum Patten and Tamas Fulop #extracellularvesicles #exosomes #MSCs #drugdelivery #neuroinflammation #Vesiculab

Engineering immunomodulatory extracellular vesicles from epithelial cells: in this recent article, Xin Luo, Raghu Kalluri at MD Anderson Cancer Center and collaborators investigated whether epithelial cell-derived EVs could be engineered to induce an immune response by modifying 293T EVs to express the immunomodulatory molecules CD80, OX40L, and PD-L1. Their engineered EVs exhibited high levels of these proteins and effectively triggered both positive and negative costimulatory signals in human and murine T cells https://lnkd.in/gSEsxwdA In cancer and autoimmune hepatitis models, they modulated T cell function and influenced disease progression. Notably, OX40L EVs enhanced antitumor activity when combined with anti-CTLA-4 in melanoma-bearing mice. Their findings demonstrate that EVs can be engineered to elicit targeted immune responses, offering translational potential for immune modulation in disease treatment. An article co-authored by Fernanda Kugeratski, Dara Dowlatshahi, Hikaru Sugimoto, Kent Arian, Yibo Fan, Li Huang, Danielle Wills, Sergio Lilla, Kelly Hodge, Sara Zanivan, Valerie LeBleu and Kathleen McAndrews #extracellularvesicles #exosomes #cancer #autoimmunity #Vesiculab

Describing prominosomes as a unique class of extracellular vesicles enriched with prominin-1/CD133: in their latest work, Jana Karbanová, Denis Corbeil at Technische Universität Dresden and collaborators explored the subcellular origins and regulatory mechanisms behind prominin-1+ extracellular vesicles (prominosomes). They examined how prominin-1 influences the release of these EVs, which may play a role in donor cell reprogramming, particularly in stem and cancer stem cells. Prominosomes facilitate intercellular communication in embryonic development and adult homeostasis while also spreading biological signals in disease states https://lnkd.in/gBfKddVg Their findings provide insights into novel molecular and cellular pathways, with potential applications in stem cell-based tissue regeneration and targeted disease interventions, especially in cancer. An article co-authored by Kristina Thamm, Christine A. Fargeas, İlker Deniz and Aurelio Lorico #extracellularvesicles #exosomes #cellsignalling #stemcell #Vesiculab

Describing how extracellular vesicles contribute to endothelial dysfunction in cardiovascular diseases: in this review article, Francisco Rafael Jimenez-Trinidad, Ana Paula Dantas at Universitat de Barcelona and collaborators provided an overview of  the evolution of extracellular vesicle research, tracing its origins from the first description in 1945 to modern insights into EV-mediated intercellular signaling and endothelial dysfunction. By analysing peer-reviewed studies from PubMed, they highlighted EVs as key regulators of cardiovascular health, capable of triggering diverse cellular responses. Their findings emphasise that EV cargo composition is dynamically influenced by factors such as sex, aging, and cardiovascular risk, shaping endothelial function and interactions with neighboring cells https://lnkd.in/ehmzUFvU They concluded that understanding the molecular mechanisms of EV–endothelium interactions could lead to novel biomarkers for cardiovascular disease monitoring and inspire advanced bioengineered therapies to improve patient outcomes. An article co-authored by Sergi Calvo, Manel Sabate, Salvatore Brugaletta, Victoria Campuzano and Gustavo Egea Guri #extracellularvesicles #exosomes #intercellularcommunication #cardiovasculardisease #Vesiculab

Developing a simplified method to isolate and characterise EVs from probiotic bacteria: in this brief report, Harshal Sawant, Ji Chen Bihl and Alip Borthakur at Marshall University evaluated ultracentrifugation and precipitation, two widely used extracellular vesicle isolation methods, to establish a standardised protocol for extracting EVs from the probiotics Lactobacillus acidophilus (LA), a Gram-positive bacterium, and Escherichia coli Nissle (EcN), a Gram-negative bacterium. Ultracentrifugation yielded approximately 1.5 times more EVs than precipitation for both strains, with EcN producing a higher EV output than LA https://lnkd.in/eNwBhWZs EVs were quantified and characterised using nanoparticle-tracking analysis (NTA) and Western blotting for surface biomarkers. They indicated that their study's primary limitation was its focus on only one Gram-positive and one Gram-negative probiotic strain, restricting broader comparisons of EV yield, size, and biomarker expression. #extracellularvesicles #bacteria #OMVs #probiotic #Vesiculab

Designing an Imaging Single Particle Profiler to analyse nanoscale bioparticles: in their latest work, Taras Sych, Erdinc Sezgin at Karolinska Institutet and collaborators developed a technology called imaging-based Single Particle Profiling (iSPP), enabling the analysis of nanoscale bioparticle such as extracellular vesicles and liposomes using standard confocal microscopy by imaging a fixed spot over time. To enhance accessibility, they developed user-friendly software with a graphical interface for quantitative analysis of particle content and properties. Using iSPP, they investigated lipid–protein interactions, drug-induced membrane modifications, and the heterogeneity of extracellular vesicles from cell lines and human urine https://lnkd.in/eYSz-jTu This widely applicable technique simplifies nanoscale bioparticle research, making advanced analysis feasible in any lab equipped with a confocal microscope. An article co-authored by André Görgens, Loïc Steiner, Gozde Gucluler Akpinar, Ylva Huge, Farhood Alamdari, Markus Johansson, Firas Al-Jabery, Amir Sherif, Susanne Gabrielsson and Samir EL Andaloussi #extracellularvesicles #exosomes #liposomes #confocalmicroscopy #Vesiculab

Delivering mRNA to the heart using EVs or LNPs: in their latest pre-print article, Nawaz Muhammad, Hadi Valadi at the University of Gothenburg and collaborators proposed that cardiac-specific extracellular vesicles could outperform non-cardiac EVs and lipid nanoparticles in delivering mRNA to the heart. In mice, intravenous administration of cardiac progenitor cell-derived EVs (CPC-EVs) demonstrated the highest efficiency in delivering modified mRNA encoding vascular endothelial growth factor A (VEGF-A) to the heart, with significantly reduced liver accumulation compared to non-cardiac EVs or LNPs https://lnkd.in/e_GhjmJm Immunofluorescence staining for CD31 and α-SMA, markers of microvascular density, revealed increased vessel density in mouse aortic rings following VEGF-A mRNA delivery via CPC-EVs. Their results highlight CPC-EVs as superior vectors for heart-specific mRNA delivery, promoting effective communication with cardiac cells, minimising off-target liver accumulation, and causing minimal transcriptomic alterations. Their findings highlight CPC-EVs as a promising platform for heart-targeted mRNA therapies. An article co-authored by Benyapa Tangruksa, Sepideh H.Hagvall, Franziska Kohl, Hernán González-King Garibotti, Yujia Jing, Zahra Payandeh (Ph.D.), Azadeh Reyahi, Karin Jennbacken, John Wiseman, Leif Hultin, Lennart Lindfors and Jane Synnergren #extracellularvesicles #LNPs #exosomes #mRNA #targeteddelivery #Vesiculab

Delivering siRNA to dorsal root ganglion neurons using lipid nanoparticles: in this pre-print article, Xiangfei (Shawn-fei） Han, JINJUN SHI at Harvard Medical School and collaborators demonstrated the use of lipid nanoparticles for effective intrathecal delivery of small interfering RNA to dorsal root ganglion (DRG) neurons, achieving potent silencing of the TRPV1 ion channel, which is predominantly expressed in nociceptor sensory neurons. This resulted in a reversible blockade of heat-, capsaicin-, and inflammation-induced nociceptive signalling, as evidenced by behavioural outcomes https://lnkd.in/eUt8kVGn Their findings highlight intrathecal siRNA LNP delivery to DRG neurons as a promising platform for identifying and validating nociceptor-selective targets, as well as for developing non-addictive treatments for post-traumatic or chronic pain caused by diabetes, nerve injury, inflammation, chemotherapy, or genetic mutations. An article co-authored by Veselina Petrova, Yudong Song, Yu-Ting Cheng, Xingya Jiang, Hui Zhou, Caleb Hu, Dean Shuailin Chen, Hyo Jeong Yong, Hyoung Woo Kim, Biyao Zhang, Omer Barkai, Aakanksha Jain, William Renthal, Philipp Lirk and Clifford Woolf #LNPs #nanoparticles #siRNA #sensoryneurons #drugdelivery #Vesiculab

Investigating extracellular vesicle-mediated intercellular epigenomic signalling: in their latest work, Kevin Yim, PhD, Ala'a Al Hrout, PhD and Richard Chahwan at the University of Zurich and EVIIVE AG utilised advanced technologies to profile B cell extracellular vesicle surface markers at the single-particle level, along with their molecular cargo and physiological roles in B cell maturation. RNA sequencing of EV non-coding RNA (ncRNA) cargo revealed a novel EV-mediated ncRNA regulatory network involved in B cell maturation. Notably, they identified a previously uncharacterised microRNA, miR-5099, alongside a group of long ncRNAs, carried within B cell EVs, potentially contributing to antibody diversification https://lnkd.in/eYXWc8VG They emphasised that EV blockade assays and complementation studies with various EV sources further validated the physiological significance and mechanism of action of EVs in driving B cell maturation. #extracellularvesicles #exosomes #intercellularsignalling #RNA #Vesiculab

Analysing small extracellular vesicles isolated from dried blood spots: in methods article, Rikke Bæk, Malene Møller Jørgensen at Aalborg University and collaborators outlined a method to extract small extracellular vesicles from dried blood spots (DBS) using Western blotting (targeting CD9, CD63, and CD81) and fluorescence nanoparticle tracking analysis (fNTA). They demonstrated that approximately 40% of sEVs could be recovered from DBS compared to direct plasma analysis. The protocol proved robust and reliable, maintaining high performance even after up to three weeks of DBS storage https://lnkd.in/eUjBhXRq Protein microarray analysis confirmed the presence of key markers, including CD9, CD63, CD81, EpCAM, Flotillin-1, CD62E/P, CD142, and CD235a. These findings highlight the potential of sEVs as diagnostic tools, supporting less-invasive, field-applicable methods suitable for vulnerable populations. An article co-authored by Jenni Sloth, Mohammad Mehedi Hasan, PhD and Getnet Balcha #extracellularvesicles #exosomes #blood #phenotyping #Vesiculab