Isolation of DNA from blood
TL Kendall, DJ Byerley, R Dean - Analytical biochemistry, 1991 - Elsevier
TL Kendall, DJ Byerley, R Dean
Analytical biochemistry, 1991•ElsevierA rapid, economical method of DNA isolation from blood was developed that yields DNA
suitable for Southern analysis and polymerase chain reactions without organic solvent
extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase
E digestion and ethanol precipitation. This isolation method readily adapts to multiple
samples. The DNA is characterized by high yield, solubility, lack of protein contamination,
and ease of restriction endonuclease digestion.
suitable for Southern analysis and polymerase chain reactions without organic solvent
extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase
E digestion and ethanol precipitation. This isolation method readily adapts to multiple
samples. The DNA is characterized by high yield, solubility, lack of protein contamination,
and ease of restriction endonuclease digestion.
A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation. This isolation method readily adapts to multiple samples. The DNA is characterized by high yield, solubility, lack of protein contamination, and ease of restriction endonuclease digestion.
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