Papers by El Sayed Ziedan
Survey of decayed banana fruits of Balady, Maghraby and Williams cultivars of local markets at Gh... more Survey of decayed banana fruits of Balady, Maghraby and Williams cultivars of local markets at Gharbeia Governorate, Egypt during 2013 and 2014 summer seasons, several postharvest diseases were observed i.e., crown rot, finger rot, neck rot and flower end rot. Routine isolation of rotten samples of banana fruits yielded mainly three genera of fungi i.e., Fusarium moniliforme J. Sheld, Thielaviopsis paradoxa (De Seynes) Höhn. and Colletotricium musae (Berk. & M.A. Curtis) Arx. Two infection types were recorded on different Cvs. of banana fruits. First infection type caused by single of each fungi, Fusarium spp., C. musae and Thialoviopsis paradoxa. Second infection type caused by Fusarium spp. + C. musae or T. paradoxa. Pathogenicity test indicated that of C. musae (No.1) was the most pathogenic fungi caused postharvest diseases on fruits of Balady Cv. followed by F. moniliforme (No.4). Pathological synergistic effect of C. musae (No.1) + F. moniliforme (No.4) on postharvest diseases...
Acta Scientific Agriculture, 2020
Root rot of grapevine an epidemic disease causing by several soil borne fungi recorded high loss ... more Root rot of grapevine an epidemic disease causing by several soil borne fungi recorded high loss plant growth of productivity. Nine endophytic biocontrol fungal and bacterial isolates of Trichoderma spp, Bacillus spp and Pseudomonas fluorescens were tested against growth of aggressive fungal isolates causing root rot of grapevine i.e. Fusarium spp. and Botryodiplodia theobromae. Results showed that the best antagonistic endophytic isolates were Trichoderma harzianum and Bacillus subtilis. Trichoderma harzianum isolate significantly reduced mycelial linear growth and conidial sporulation of fungi tested as well as over lapping growth of fungi tested. In addition, Bacillus subtilis recorded high zone inhibition toward all pathogenic fungi tested than another bacterial isolates in this study. Some promising essential oils, organic, antioxidants acids and their combinations were tested on growth of endophytic biocontrol agents of Trichoderma harzianum and Bacillus subtilis. Results obtained indicated that sorbic acid followed by propionic acid were the least effect on mycelial linear growth and conidial sporulation of Trichoderma harzianum. In addition, sorbic acid, propionic acid (0.1%), black seed oil (4.0%) and their combination had no effect negatively on Bacillus subtilis growth. In greenhouse experiment soil drench by propionic + sorbic acids, propionic acid + sorbic acid + T. harzianum + B. subtilis were the best and significantly treatments its completely suppress (100%) root rot disease percentage and disease severity on shoots and roots of grapevine plants followed by T. harzianum as individual or combination with B. subtilis were increased morphological characters of grapevine plant i.e. length, fresh and dry weight of and root size. So, combined soil treatment by propionic acid + sorbic acid or propionic acid + sorbic acid + T. harzianum + B. subtilis as the safe fungicide alternatives for management root rot disease of grapevine.
Manipulating endophytic bacteria for biological control to soil borne diseases of peanut. J Appl Sci Res 2:497-502
Effect of salicylic acid on Fusarium oxysporum and their pathological potential on cowpea Effect of salicylic acid on Fusarium oxysporum and their pathological potential on cowpea
Pathogenic isolates of Fusarium oxysporum were isolated from roots of cowpea plants naturally inf... more Pathogenic isolates of Fusarium oxysporum were isolated from roots of cowpea plants naturally infected by root-rot disease. Soaking cowpea seeds (Cv. Giza 331) on different concentrations of salicylic acid for one hour was increasing percentage of seed germination, reduced diseased germinated seed and significantly reduced Fusarium spp. colonization on cowpea germinated seed. These effects were increased by increasing concentrations of salicylic acid up to 20 µM/ ml. In vitro salicylic acid was observed as fungicidal effect on the linear growth of three isolates of F. oxysporum on dual culture after amended by different salicylic acid concentrations i.e., 0,1,2,4,5,10 and 20 M/ml ,fungicidal effect on fungal linear growth was increased by increasing concentrations. Twenty M/ml of salicylic acid was completely suppressing linear growth of fungal isolates. Also, in liquid culture of potato dextrose broth medium , the activities of cell wall degrading enzymes i.e., pectineases, prote...
Ziedan, E.H.; Mostafa, M.H Elewa ,I. .. (2012) Effect of bacterial inocula on Fusarium oxysporum f. sp. sesami and their pathological potential on sesame
Fusarium oxysporum f.sp. sesami (Zap) Cast. causing wilt disease of sesame plants causing high lo... more Fusarium oxysporum f.sp. sesami (Zap) Cast. causing wilt disease of sesame plants causing high losses in quality and quantity of sesame seed yield.Bacterial isolates of Bacillus megatherium and Azospirillium brasilense were the main biological component respectively of commerical biofertilizers i.e., phosphoren and cerialin respectively in Egypt .These bacteria were reduced linear growth and conidiospores production of Fusarium oxysporum f.sp. sesami their effects were increased in combination with fungicide (Topsin) which increased by increasing fungicide concentration . Futhermore, several morphological changes of fungal hyphae by Bacillus megatherium stress. In laboratory, plate culture of Fusarium oxysporum f.sp. sesami was used for evaluate ability of i.e., cerialin, phosphoren, rhizobacterin and blue green algae as well as fungicide (Topsin M-70 %) for protection sesame seedlings from Fusarium blight .All different commercial biofertilizers and Topsin were significantly protec...
Ziedan E.H.E. (2013) . First report of pod blight of okra caused by Choanephora cucurbitarum in Egypt
During May and June 2010, in Kafr El –Abiada village,El Mahalla El Kobera District, Gharbeia Gove... more During May and June 2010, in Kafr El –Abiada village,El Mahalla El Kobera District, Gharbeia Governorate, Egypt. Okra plants were exhibited blossom blight, chlorosis and wet rotten of young fruiting pods. The high losses of fresh pods was observed due to soften wet rot of young pods during their developing. Diseased pods fail to develop during a period of hot and high humidity conditions. Under highly humidity degree in damping chamber, diseased young pods was covered with a cottony growth. Isolation trails from diseased tissue of diseased young pods on potato dextrose agar medium (PDA) revealed that several fungal isolates. A white aerial mycelia that later developed light yellow was identified as Choanephora cucurbitarum (Berk&Ravenel) Thaxt. On the basis of cultural and morphological characteristics. Pathogenicity tests were performed by inoculation young pods by mycelial mat of fungal isolates from 7- to 10-day-old cultures grown on (PDA). Pods free inoculation was served as a c...
Eman S. H. Farrag , Moustafa H. A. Moharam, and El-Sayed H. Ziedan ( 2013). Effect of plant extracts on morphological and pathological potential of seed-borne fungi on cucumber seeds
Seed-borne fungi of cucumber are serious problem worldwide causing damping-off, root rot and wilt... more Seed-borne fungi of cucumber are serious problem worldwide causing damping-off, root rot and wilt diseases on cucumber plants. Several pathogenic fungal isolates were isolated from cucumber seed samples collected from commercial markets in Egypt. Fusarium oxysporum and Fusarium solani were the common fungi isolated from cucumber seeds followed by Alternaria sp, Rhizoctonia solani, Helminthosporium sp. and Penicillium spp. Pathogenicity test indicated that F. oxysporum was the best fungal significantly induced damping off on cucumber plants. Water extract of peppermint extract was the most effective completely inhibited spore germination and mycelial growth of F. oxysporum at concentration of 2%, followed by rheum and garlic extracts which completely inhibited fungal conidiospore germination and mycelial growth on agar medium by the rate of 3%. Cucumber seeds treated with 2% peppermint extract caused a highly reduction of damping off of cucumber and reduced fungal transmission from s...
Chemical fumigants as alternatives methyl bromide for soil disinfestation of plant integrated pest management
International Journal of Agricultural Technology, 2016
Journal of Plant Protection Research
This work was carried out during two successive seasons (2016 and 2017) on cucumber fruits from a... more This work was carried out during two successive seasons (2016 and 2017) on cucumber fruits from a plastic greenhouse and from open field cultivation in El Gharbeia and El Giza Governorates, Egypt. Isolation trials from spoilage fruit samples of plastic greenhouse cultivation recorded high frequency of Alternaria tenusinium, Fusarium spp. and Pleospora alli. The most common fungi of rotten cucumber fruits from an open field were Galactomyces spp. and Fusarium spp. Pathogenicity tests proved that, Fusarium solani from El-Gharbeia followed by A. tenusinium from El-Giza were the most frequent isolates responsible for rot of cucumber fruits from plastic greenhouse cultivation. Moreover, the most frequent isolates causing postharvest disease of cucumber fruits of the open field were Galactomy ces candidium from El-Giza followed by Geotrichum sp. and F. fujikuroi from El-Gharbeia Governorates, respectively. This is the first report of several fungi causing postharvest fruit rot disease of cucumber i.e., G. candidium, Geotrichum sp., A. tenusinium, P. alli and Fusar ium spp. (F. fujikuroi, F. verticiolides, F. solani, F. geraminearium and Fusarium incarna tum). Fungal isolates were identified according to cultural, morphological and molecular characterization based on sequencing of internal transcribed spacer1 (ITS1). All the ITS nucleotide sequences of fungi were applied and conserved in GenBank.
Journal of Plant Protection Research
This study was executed to investigate the potential of agar-agar, a nontoxic and non-degradable ... more This study was executed to investigate the potential of agar-agar, a nontoxic and non-degradable gelling agent, as a promising coating agent to improve and protect banana fruit against fungal postharvest diseases i.e., crown, finger, neck and flower end rots which are caused by fungal isolates of Colletotrichum musae and Fusarium moniliforme. Coated-banana fruit samples with different concentrations of agar-agar suspension particularly at 2.0 g • l −1 exhibited a significant reduction in incidence and severity of postharvest diseases compared to untreated fruit. Banana fruits dipped in agar suspension at 2.0 g • l −1 for 5, 10 and 15 min showed significant reduction in disease incidence and severity. Moreover, application of agar suspension as a coating agent at 2.0 g • l −1 significantly decreased weight loss (%), firmness loss (%), and soluble solid concentration of banana fruit for 15 days at 25 ± 2°C. Scanning electron microscopy observation confirmed that the fruit coated with agar colloid at 2.0 g • l −1 had significantly fewer cracks and showed smoother surfaces than untreated fruit. This explains the quality improvement in agar-coated fruit compared to uncoated fruit. Overall, agar colloid, a safe coating agent, could be used to protect banana fruit against postharvest rot diseases and extend fruit storage life during ripening and storage.
Agriculture
This research evaluated the efficacy of essential oils in the management of cucumber powdery mild... more This research evaluated the efficacy of essential oils in the management of cucumber powdery mildew. Essential oils of lemongrass, lemon, thyme, peppermint, abundance blend, purification blend, and thieves blend were tested in vitro and under greenhouse conditions in two separate experiments. The effects of essential oils were tested against powdery mildew disease at concentrations of 1.0–2.5 mL/L, and the consequent impact of the oils on plant growth was evaluated. Powdery mildew fungus, Podosphaera xanthii, was identified using sequencing of the ITS region. The essential oils significantly reduced disease incidence up to 77.3% compared with the positive control (p < 0.5). Moreover, the essential oils increased the plant length (up to 187 cm), leaf area (up to 27.5 cm2), fresh weight (up to 123 g), dry weight (up to 22.5 g), number of flowers (16.3), and metabolite content compared with the positive control (p < 0.5). Cell membrane injury decreased significantly in the oil-tr...
Application of composts and potassium sulphate on root rot incidence, morphological growth, yield components, oil content and constitutes of marjoram plants (Majorana hortensis L.)
Biocatalysis and Agricultural Biotechnology, 2022
Biological control of grapevine root-rot by antagonistic microorganisms
The integrative management of banana postharvest diseases i.e., crown, neck, finger and flower en... more The integrative management of banana postharvest diseases i.e., crown, neck, finger and flower end rots caused by Colletotrichum musae (Berk & M.A.Curtis ) Arx and Fusarium moniliforme J. Sheld was carried out by dipping banana fruits in each suspensions for 10 minutes of clove oil (2.0%), agar suspension (0.2%) and their combinations compared with hot water and untreated fruits. Results declared that all dipping treatments significantly reduced the incidence and severity of all postharvest rot diseases in banana fruits compared with untreated fruits. Interestingly, the single application of clove oil (2.0%) or agar suspension (0.2%) were the most effective and significants treatments reduced the incidence and severity of postharvest rot diseases. However, no significant effects on fruit firmness loss (%) and soluble solid concentration (SSC) in treated fruits and untreated. Meanwhile, individual application of each clove oil (2.0%) or agar suspension (0.2%) greatly decreased fruit ...
Plant Archives, 2018
Survey of fruits blight (grey mould disease) on fruits of cucumber plants cultivations during gro... more Survey of fruits blight (grey mould disease) on fruits of cucumber plants cultivations during growing winter season 2016 2017 in protective plastic houses of some Governorates in Egypt indicated that fruits blight is the most epidemic foliar disease of cucumber. Botrytis cinerea was the common fungi of diseased fruits at El-Beheira followed by ElGharbeia Governorates respectively. Pathogenicity test of fungal isolates revealed that Botrytis cinerea isolates (No. 5 and 2) respectively which isolated from ElGharbeia caused highly incidence of fruit rot of cucumber followed by isolate (No.7) from E Beheira Governorate. Fungal isolates were identified as Botrytis cinerea according to cultural, morphological and molecular characterizations based on sequencing of internal transcribed space (ITS). Some nucleotides sequences were registries of fungi in Gene Bank under accession number of MF996362, MF996363, MF996364, MF996365, MF996366, MF996367 and MF996368.
Molecular characterization of Alcaligenes faecalis and Pseudomonas aeruginosa causing root rot of date palm
International Journal of Agriculture and Biology, 2020
International Journal of Agricultural Technology, 2013
Seed-borne fungi of cucumber are serious problem worldwide causing damping-off, root rot and wilt... more Seed-borne fungi of cucumber are serious problem worldwide causing damping-off, root rot and wilt diseases on cucumber plants. Several pathogenic fungal isolates were isolated from cucumber seed samples collected from commercial markets in Egypt. Fusarium oxysporum and Fusarium solani were the common fungi isolated from cucumber seeds followed by Alternaria sp, Rhizoctonia solani, Helminthosporium sp. and Penicillium spp. Pathogenicity test indicated that F. oxysporum was the best fungal significantly induced damping off on cucumber plants. Water extract of peppermint extract was the most effective completely inhibited spore germination and mycelial growth of F. oxysporum at concentration of 2%, followed by rheum and garlic extracts which completely inhibited fungal conidiospore germination and mycelial growth on agar medium by the rate of 3%. Cucumber seeds treated with 2% peppermint extract caused a highly reduction of damping off of cucumber and reduced fungal transmission from s...
Increasing the efficacy of Trichoderma harzianum as biocontrol agent by slow release nitrogen fertilizers for wilt control of sunflower
Industrial Crops and Products, 2021
This study demonstrates the feasibility and efficacy of using flow cytometric analysis with intra... more This study demonstrates the feasibility and efficacy of using flow cytometric analysis with intracellular cytokine staining for characterization of T-cell phenotype and functional status in extensive alopecia areata (EAA) scalp skin. Cell suspensions were made from scalp punch biopsies taken from 12 patients with long-standing EAA (average disease duration 14 years, 95% hair loss) and six control subjects. EAA samples had a lower percentage of CD-3-expressing cells, but CD-4/CD-8 ratios remained similar to controls. Expression of CD-69 was found only in EAA scalp biopsies, suggesting that T-cells from EAA scalp have undergone activation. No difference was found in tumor necrosis factor a expression. Surprisingly, EAA scalp T-cells produced less IL-2 and CD-8 T-cells produced less IFN-g. Immunohistochemical staining of formalin-fixed paraffin-embedded specimens demonstrated that IFN-g-producing cells in EAA scalp were not greater in number than in normal specimens. The few identified IFN-g-producing cells demonstrated no tendency to localize to the perifollicular region, and were similarly distributed as in control specimens. The abnormalities in cytokine production may explain the relative paucity of inflammatory change observed in the clinical setting and suggest that T-cell responses in EAA scalp are tightly, albeit aberrantly, regulated via mechanisms of peripheral T-cell tolerance.
GSC Biological and Pharmaceutical Sciences, 2020
Pathogenic soil borne fungi causing epidemic root rot disease on grapevine and high loss of plant... more Pathogenic soil borne fungi causing epidemic root rot disease on grapevine and high loss of plant growth and fruit yield. Fast detection of aggressive fungal isolates is the first tool for introduce selection effective a agent for management disease progress. In vitro two simple techniques based on stem cutting of grapevine i.e., mycelial growth and cell wall degrading enzymes activities were evaluated for screening of aggressive fungal isolates in comparsion with classical pathogenicity test. Fourteen isolates of fungi associated with root rot disease of grapevine plants i.e. Fusarium oxysporumi Schlechtend, Fusarium solani (Mart.) Sacc, Botryodiplodia theobromae, Rhizoctonia solani Kuhn and Macrophomina phaseolina (Tassi) Gold. were tested and pathological action on grapevine plant (Cv.crimson) under soil artificially infested by classical method with their mycelial growth,cell wall degrading enzymes activities on stem cutting of grapevine as well as each fungal isolates of shoot and root compare the control. Fungal isolate of Botryiodiplodia theobromae No. (7) was the most isolate recorded high and significant root rot (100%) incidence and disease severity on grapevine plants followed by Fusarium avenacum isolate No. (11) and Fusarium solani isolate No. (12). Positive correlation coefficient was observed between mycelial growth of fungal isolates tested on stem cutting of grapevine plants and root rot disease incidence and their disease severity followed by their production pectinase and cellulase enzymes. So, mycelial of fungal growth on stem cutting of grapevine is a good and simple of opportunity technique for determination aggressive degrees of fungal isolates in vitro as alternative accuracy, very short time test and very shape cost than classical pathogenicity test.
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Papers by El Sayed Ziedan