Papers by manuel De Rojas
For a long time, classification of Demodex mites has been based mainly on their hosts and phenoty... more For a long time, classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics. A new subspecies of Demodex folliculorum has been proposed, but not confirmed. Here, cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D. folliculorum. Sequencing showed that the mitochondrial cox1 fragments of five D. folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%. The average sequence divergence was 1.24% among the five Chinese isolates, 0.94% between the two geographical isolate groups (China (5) and Spain (1)), and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)). The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125, 2.7 (3/1) among the five Chinese isolates, 0.0094, 3.1 (3/1) between the two geographic...
A phylogeographic study was carried out of Trichuris muris, nematode parasitizing Murinae rodents... more A phylogeographic study was carried out of Trichuris muris, nematode parasitizing Murinae rodents from the Muridae family, isolated from four different hosts and from different geographical regions of Europe by amplification and sequencing of the ITS1-5.8S-ITS2 fragment of the ribosomal DNA. T. muris was found in the Apodemus sylvaticus, Apodemus flavicollis, Mus domesticus, and Rattus rattus rodents. The molecular results confirm the presence of DNA polymorphisms among T. muris isolates from Europe. The present study shows two clear-cut geographical and genetic lineages: one of them is widespread from northern Spain (Catalonia) to Denmark (Western European region), while the second is widespread in the Eastern European region (Croatia, Rumania, and Turkey). These two genotypes can be easily distinguished by a PCR-RFLP analysis of this sequence with the ApalI restriction enzyme. Moreover, networks and phylogenetic reconstructions also reveal that T. muris from various Murinae rodent...
Genome-resolved metagenomic analyses reveal the presence of a bacterial endosymbiont in an avian nasal mite (Rhinonyssidae; Mesostigmata)
Rhinonyssidae (Mesostigmata) is a family of nasal mites only found in birds. All species are hema... more Rhinonyssidae (Mesostigmata) is a family of nasal mites only found in birds. All species are hematophagous endoparasites, which may damage the nasal cavities of birds, and also could be potential reservoirs or vectors of other infections. However, the role of members of Rhinonyssidae as disease vectors in wild bird populations remains uninvestigated, with studies of the microbiomes of Rhinonyssidae being almost non-existent. In the nasal mite (Tinaminyssus melloi) from rock doves (Columba livia), a previous study found evidence of a highly abundant putatively endosymbiotic bacteria from Class Alphaproteobacteria. Here, we expanded the sample size of this species, incorporated contamination controls, and increased sequencing depth in shotgun sequencing and genome-resolved metagenomic analyses. Our goal was to increase the information regarding this mite species with its putative endosymbiont. Our results support the endosymbiotic nature of this bacterial taxon, which is the first des...
Although there is an absence of common guidelines or regulations about wastewater reuse at Europe... more Although there is an absence of common guidelines or regulations about wastewater reuse at European Community level, there are several countries or federal regions that have published their own standards or regulations. In Spain, the current Royal Decree 1620/2007 regulates the legal regime for reuse of treated wastewaters for different uses. The aim of present study is to evaluate the removal performance of certain parasitic and pathogenic organisms by means of the use of different extensive wastewater treatment technologies in small communities. The study results were assessed based on both the EU Urban Wastewater Directive (91/271/EEC) and Spanish Royal Decree. The selected extended technologies are installed in the Experimental Plant of Urban Wastewater Treatment of Carrion de los Cespedes, PECC, (Sevilla, Andalusia, Spain) property of Andalusian Water Agency (Andalusian Department of the Environment). These extensive technologies are: 1) stabilization ponds, 2) constructed wetl...
Trichuris colobae n. sp. (Nematoda: Trichuridae), a new species of Trichuris from Colobus guereza kikuyensis
In the present work, a morphological and biometrical study of whipworms Trichuris Roederer, 1761 ... more In the present work, a morphological and biometrical study of whipworms Trichuris Roederer, 1761 (Nematoda: Trichuridae) parasitizing Colobus guereza kikuyensis has been carried out. Biometrical and statistical data showed that the mean values of individual variables between Trichuris suis and Trichuris sp. from C. g. kikuyensis differed significantly (P < 0.001) when Student’s t test was performed: seven male variables (width of esophageal region of body, maximum width of posterior region of body, width in the place of junction of esophagus and the intestine, length of bacillary stripes, length of spicule, length of ejaculatory duct, and distance between posterior part of testis and tail end of body) and three female variables (width of posterior region of body, length of bacillary stripes, and distance of tail end of body and posterior fold of seminal receptacle). The combination of these characters permitted the discrimination of T. suis with respect to Trichuris sp. from C. g...
Diversity
Rhynonyssidae is a family of endoparasitic hematophagous mites, which are still largely unknown e... more Rhynonyssidae is a family of endoparasitic hematophagous mites, which are still largely unknown even though they could act as vector or reservoir of different pathogens like dermanyssids. Sampling requirements have prevented deeper analysis. Rhinonyssids have been explored in a few host specimens per species, leading to undetailed morphological descriptions and inaccurate epidemiology. We explore the relationships established between these parasites in two Columbiformes urban birds (domestic pigeon (Columba livia domestica) and Eurasian collared dove (Streptopelia decaocto)), assesing 250 individuals of each type in Seville (Spain). As expected, Mesonyssus melloi (Castro, 1948) and Mesonyssus columbae (Crossley, 1950) were found in domestic pigeons, and Mesonyssus streptopeliae (Fain, 1962) in Eurasian collared doves. However, M. columbae was found for the first time in Eurasian collared doves. This relationship could be common in nature, but sampling methodology or host switching c...
Complete Mitochondrial Genomes and Bacterial Metagenomic Data From Two Species of Parasitic Avian Nasal-Mites (Rhinonyssidae: Mesostigmata)
Frontiers in Ecology and Evolution
Background Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory trac... more Background Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory tract. There are around 600 species described worldwide and almost all species of birds are found to have embedded rhinonyssid mites. Despite their presumed relevance, these mites are largely unstudied due to the difficulty in sampling them and, therefore, the majority of mite-host associations and species-prevalence data are unknown. New information In this study, 179 mite specimens belonging to 27 species and eight genera were identified. Notably, 18 new mite-bird associations were documented for the first time, thus increasing the known host range for these mite species. In addition, mite-host associations found in ‡ § | © De Rojas M et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. this study were compared with known associations from these species of birds in the European part of Russia and in Europe. Overall, this study represents the largest survey to date carried out on rhinonyssid mites in Russia and one of the most comprehensive datasets on rhinonyssid host-range.
Background Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory trac... more Background Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory tract. There are around 600 species described worldwide and almost all species of birds are found to have embedded rhinonyssid mites. Despite their presumed relevance, these mites are largely unstudied due to the difficulty in sampling them and, therefore, the majority of mite-host associations and species-prevalence data are unknown. New information In this study, 179 mite specimens belonging to 27 species and eight genera were identified. Notably, 18 new mite-bird associations were documented for the first time, thus increasing the known host range for these mite species. In addition, mite-host associations found in ‡ § | © De Rojas M et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. this study were compared with known associations from these species of birds in the European part of Russia and in Europe. Overall, this study represents the largest survey to date carried out on rhinonyssid mites in Russia and one of the most comprehensive datasets on rhinonyssid host-range.
Background Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory trac... more Background Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory tract. There are around 600 species described worldwide and almost all species of birds are found to have embedded rhinonyssid mites. Despite their presumed relevance, these mites are largely unstudied due to the difficulty in sampling them and, therefore, the majority of mite-host associations and species-prevalence data are unknown. New information In this study, 179 mite specimens belonging to 27 species and eight genera were identified. Notably, 18 new mite-bird associations were documented for the first time, thus increasing the known host range for these mite species. In addition, mite-host associations found in ‡ § | © De Rojas M et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. this study were compared with known associations from these species of birds in the European part of Russia and in Europe. Overall, this study represents the largest survey to date carried out on rhinonyssid mites in Russia and one of the most comprehensive datasets on rhinonyssid host-range.
Biodiversity Data Journal
Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory tract. There ar... more Rhinonyssid mites are permanent parasites of birds that inhabit their respiratory tract. There are around 600 species described worldwide and almost all species of birds are found to have embedded rhinonyssid mites. Despite their presumed relevance, these mites are largely unstudied due to the difficulty in sampling them and, therefore, the majority of mite-host associations and species-prevalence data are unknown. In this study, 179 mite specimens belonging to 27 species and eight genera were identified. Notably, 18 new mite-bird associations were documented for the first time, thus increasing the known host range for these mite species. In addition, mite-host associations found in this study were compared with known associations from these species of birds in the European part of Russia and in Europe. Overall, this study represents the largest survey to date carried out on rhinonyssid mites in Russia and one of the most comprehensive datasets on rhinonyssid host-range.
Evidence of cryptic species in the genus Tinaminyssus (Acari: Rhinonyssidae) based on morphometrical and molecular data
Experimental & applied acarology, Jan 6, 2018
The study of cryptic species allows to describe and to understand biodiversity, and the evolution... more The study of cryptic species allows to describe and to understand biodiversity, and the evolutionary processes shaping it. Mites of the family Rhinonyssidae are permanent parasites of the nasal cavities of birds, currently including about 500 described species and 12 genera. Here, we tested the hypothesis that mites from five populations of the genus Tinaminyssus-three isolated from European turtle doves (Streptopelia turtur), and two from Eurasian collared doves (Streptopelia decaocto; Aves: Columbiformes)-are, in fact, two cryptic species inhabiting different hosts. First, we performed a morphometrical study on 16 traits. Then, we used the ITS1-5.8S rDNA-ITS2 nuclear region (ITS region), and a fragment of the mitochondrial cytochrome c-oxidase 1 (COI) to carry out phylogenetic and species delimitation analyses on Tinaminyssus species. Morphological analyses revealed a lack of biometric differentiation among Tinaminyssus populations from the two host species. However, molecular ana...
Morphological and molecular study of the genus Nosopsyllus (Siphonaptera: Ceratophyllidae). Nosopsyllus barbarus (Jordan & Rothschild 1912) as a junior synonym of Nosopsyllus fasciatus (Bosc d’Antic, 1800)
Insect Systematics & Evolution
In the present work, a comparative morphological and molecular study of Nosopsyllus barbarus (Jor... more In the present work, a comparative morphological and molecular study of Nosopsyllus barbarus (Jordan & Rothschild 1912) and Nosopsyllus fasciatus (Bosc, d’Antic 1800) isolated from rodents from different geographical regions (Spain, Morocco, Belgium, France and Portugal) has been carried out. The Internal Transcribed Spacers 1 and 2 (ITS1, ITS2) and partial 18S rRNA, and partial cytochrome c oxidase 1 (cox1) and cytochrome b (cytb) mtDNA sequences were determined to clarify the taxonomic status of these two species and to assess intraspecific variation and inter-specific sequence differences. In addition, a phylogenetic analysis with other species of fleas using Bayesian inference was performed. We have found morphological differences between N. barbarus and N. fasciatus that did not correspond with molecular differences. Furthermore, any of the five molecular markers used in this study was able to discriminate between the two species. Thus, based on the phylogenetic and molecular s...
Cytochrome oxidase subunit 1 and mitochondrial 16S rDNA sequences of Trichuris skrjabini (Tricocephalida: Trichuridae)
Parasitol Res, 2009
The partial mitochondrial cytochrome c-oxidase subunit 1 gene (cox 1) and partial mitochondrial 1... more The partial mitochondrial cytochrome c-oxidase subunit 1 gene (cox 1) and partial mitochondrial 16S ribosomal DNA of Trichuris skrjabini (Baskakov 1924) isolated from Capra hircus have been amplified and sequenced. The analyses of multiple sequence alignments of mitochondrial 16S rDNA and cox 1 of T. skrjabini revealed high homology with those of Trichinella species. For the first time, the mitochondrial DNA gene sequences of one species of trichurid nematode have been cited.
Ctenocephalides felisandCtenocephalides canis: introgressive hybridization?
Systematic Entomology, 2016
Boletin De La Real Sociedad Espanola De Historia Natural Seccion Biologica, 2003
Revista ibérica de parasitología
Revista Española de Salud Pública
La\ paraslto& intestinales en Io? niño\ constituyen un problema de salud púbhca que debe ser valo... more La\ paraslto& intestinales en Io? niño\ constituyen un problema de salud púbhca que debe ser valorado periódicamente en cada reglón. En e\te ttabajo se aborda. por primera 1 ez en la región natural del C'alle tlel Guadalquivir. un e\tudin amplio sobre la prekalencla del parasitkmo mte\tinal en la población Infantil de la zona. >Gtodos: Durante el período 1994 1996. mediante análik coprológtco 1 método de Graham. 4e ha e\tudlado a 1.917 niño\ ! mña\ asintomático<. con edade\ comprendidas entre \elk 1 diez año\. residentes en 1 einte localidades del Valle del Guadalquwir. Conclusiones: La pre\ alencla global encontrada e\ 4mtlar LL 1~ de otra\ regwne\ española\. aunque yuizá\ pueda constdewrw al+~ más fa\xable No w detectan peoh~lminto~. jebido po4lemente :I !a mejor3 de 1;: mfrae\tru¿tur:i higiénico-\anitari,l ! ;I los etectos de lu prolongada aquí;, en la zona. La wardiws. a diferencta de Ia\ re\tante5 protozoosls. mantiene = una prevalcncia relattk amente alta.
Molecular study of Stenoponia tripectinata tripectinata (Siphonaptera: Ctenophthalmidae: Stenoponiinae) from the Canary Islands: taxonomy and phylogeny
Bulletin of Entomological Research, 2015
In the present work, we carried out a comparative molecular study ofStenoponia tripectinata tripe... more In the present work, we carried out a comparative molecular study ofStenoponia tripectinata tripectinataisolated fromMus musculusfrom the Canary Islands, Spain. The Internal Transcribed Spacers 1 and 2 (ITS1, ITS2) and 18S ribosomal RNA partial gene and cytochrome c-oxidase 1 (cox1) mitochondrial DNA partial gene sequences of this subspecies were determined to clarify the taxonomic status of this subspecies and to assess inter-population variation and inter-specific sequence differences. In addition, we have carried out a comparative phylogenetic study with other species of fleas using Bayesian, Maximum Parsimony, Maximum Likelihood and Neighbor-Joining analysis. A geographical signal was detected between thecox1partial gene sequences ofS. t. tripectinataisolated fromM. musculusfrom different islands and those isolated fromApodemus sylvaticusfrom the Iberian Peninsula. Our results assess the monophyletic origin of Stenoponiinae and a different genetic lineage from Ctenophthalmidae. ...
Boletín de la Real Sociedad Española de Historia Natural. Sección biológica
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Papers by manuel De Rojas