eprintid: 77173
rev_number: 8
eprint_status: archive
userid: 16805
dir: disk0/00/07/71/73
datestamp: 2013-03-26 14:08:48
lastmod: 2021-09-23 08:37:11
status_changed: 2013-03-26 14:08:48
type: article
metadata_visibility: show
creators_name: Rodríguez Hernández, J.C.
creators_name: Salmerón-Sánchez, M.
creators_name: Soria, J.M.
creators_name: Gómez Ribelles, J.L.
creators_name: Monleón Pradas, M.
creators_orcid: 0000-0002-8112-2100
title: Substrate chemistry-dependent conformations of single laminin molecules on polymer surfaces are revealed by the phase signal of atomic force microscopy
ispublished: pub
divisions: 30303000
abstract: The conformation of single laminin molecules adsorbed on synthetic substrates is directly observed making use of the phase magnitude in tapping mode atomic force microscopy (AFM). With AFM, it is not possible to differentiate the proteins on the substrate if use is made of the height signal, since the roughness of the material becomes of the same order of magnitude as the adsorbed protein, typically 10 nm height. This work shows how AFM can be exploited to reveal protein conformation on polymer materials. Different laminin morphologies are observed on a series of different copolymers based on ethyl acrylate and hydroxyethyl acrylate as a function of the surface density of −OH groups: from globular to completely extended morphologies of the protein molecules are obtained, and the onset of laminin network formation on some substrates can be clearly identified. The results stress the importance of the underlying synthetic substrate’s surface chemistry for the biofunctional conformation of adsorbed proteins.
date: 2007-07-01
date_type: published
id_number: 10.1529/biophysj.106.102491
uniqueid: glaseprints:2007-77173
issn_online: 1542-0086
scopus_impact: 24
scopus_cluster: 2-s2.0-34447269885
scopus_datestamp: 2013-10-26 09:33:21
wos_impact: 18
wos_cluster: WOS:000247061500023
wos_datestamp: 2013-05-21 23:21:00
full_text_status: none
publication: Biophysical Journal
volume: 93
number: 1
pagerange: 202-207
refereed: TRUE
issn: 0006-3495
hoa_compliant: 305
hoa_date_pub: 2007-07-01
hoa_exclude: FALSE
hoa_gold: FALSE
citation:    Rodríguez Hernández, J.C., Salmerón-Sánchez, M. <https://eprints.gla.ac.uk/view/author/30067.html>, Soria, J.M., Gómez Ribelles, J.L. and Monleón Pradas, M.  (2007)  Substrate chemistry-dependent conformations of single laminin molecules on polymer surfaces are revealed by the phase signal of atomic force microscopy.   Biophysical Journal <https://eprints.gla.ac.uk/view/journal_volume/Biophysical_Journal.html>, 93(1),  pp. 202-207.   (doi: 10.1529/biophysj.106.102491 <https://doi.org/10.1529/biophysj.106.102491>)