eprintid: 76784
rev_number: 10
eprint_status: archive
userid: 16805
dir: disk0/00/07/67/84
datestamp: 2013-03-25 11:25:20
lastmod: 2021-09-23 08:33:30
status_changed: 2013-03-25 11:25:20
type: article
metadata_visibility: show
creators_name: Costa Martínez, E.
creators_name: Escobar Ivirico, J.L.
creators_name: Muñoz Criado, I.
creators_name: Gómez Ribelles, J.L.
creators_name: Monleón Pradas, M.
creators_name: Salmerón-Sánchez, M.
creators_orcid: 0000-0002-8112-2100
title: Effect of poly(L-lactide) surface topography on the morphology of in vitro cultured human articular chondrocytes
ispublished: pub
divisions: 30303000
abstract: Human articular chondrocytes were cultured in vitro on poly(L-lactic) acid, PLLA, substrates. Influence of the surface topography on cell morphology was found. Different surface microtopographies were obtained on PLLA by crystallizing at 120 °C after nucleation treatments that include isothermal stages at temperatures just below (55 °C) and just above (75 °C) the glass transition temperature (T <sub>g</sub>  = 65 °C). Isothermal crystallization from the melt gave rise to big spherulites (approx. 50 μm diameter) with approx. 1 μm depth. Crystallization after nucleation treatments results in smaller (approx. 5 μm)—difficult to distinguish—spherulites. Cell viability was excellent and not affected by the surface roughness. Cell population on the nucleated samples resembles the result of culture on the reference tissue culture polystyrene (TCPS). However, cells cultured on big spherulites (PLLA isothermally crystallized without nucleation treatment) show a peculiar morphology, with a more isolated disposition and growth oriented in a characteristic direction.
date: 2007-08
date_type: published
id_number: 10.1007/s10856-007-3038-1
uniqueid: glaseprints:2007-76784
published_online: 2007-05-05
issn_online: 1573-4838
scopus_impact: 11
scopus_cluster: 2-s2.0-34447120183
scopus_datestamp: 2013-10-26 09:27:50
wos_impact: 10
wos_cluster: WOS:000248226900020
wos_datestamp: 2013-05-21 22:55:01
full_text_status: none
publication: Journal of Materials Science: Materials in Medicine
volume: 18
number: 8
pagerange: 1627-1632
refereed: TRUE
issn: 0957-4530
hoa_compliant: 305
hoa_date_pub: 2007-05-05
hoa_exclude: FALSE
hoa_gold: FALSE
citation:    Costa Martínez, E., Escobar Ivirico, J.L., Muñoz Criado, I., Gómez Ribelles, J.L., Monleón Pradas, M. and Salmerón-Sánchez, M. <https://eprints.gla.ac.uk/view/author/30067.html>  (2007)  Effect of poly(L-lactide) surface topography on the morphology of in vitro cultured human articular chondrocytes.   Journal of Materials Science: Materials in Medicine <https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Materials_Science=3A_Materials_in_Medicine.html>, 18(8),  pp. 1627-1632.   (doi: 10.1007/s10856-007-3038-1 <https://doi.org/10.1007/s10856-007-3038-1>)