eprintid: 76767 rev_number: 11 eprint_status: archive userid: 16805 dir: disk0/00/07/67/67 datestamp: 2013-03-20 12:28:58 lastmod: 2021-09-23 08:33:16 status_changed: 2013-03-20 12:28:58 type: article metadata_visibility: show creators_name: Escobar Ivirico, J.L. creators_name: Salmerón-Sánchez, M. creators_name: Gómez Ribelles, J.L. creators_name: Monleón Pradas, M. creators_name: Soria, J.M. creators_name: Gomes, M.E. creators_name: Reis, R.L. creators_name: Mano, J.F. creators_orcid: 0000-0002-8112-2100 title: Proliferation and differentiation of goat bone marrow stromal cells in 3D scaffolds with tunable hydrophilicity ispublished: pub divisions: 30303000 abstract: We have synthesized methacrylate-endcapped caprolactone networks with tailored water sorption ability, poly(CLMA-co-HEA), in the form of three-dimensional (3D) scaffolds with the same architecture but exhibiting different hydrophilicity character (x<sub>HEA</sub>=0, 0.3, 0.5), and we investigated the interaction of goat bone marrow stromal cells (GBMSCs) with such structures. For this purpose, GBMSCs were seeded and cultured for 3, 7, 14, 21, and 28 days onto the developed scaffolds. Cells have proliferated throughout the whole scaffold volume. Cell adhesion and morphology were analyzed by SEM, whereas cell viability and proliferation was assessed by MTS test and DNA quantification concluding that numbers of cells increased as a function of the culturing time (until day 14) and also with the hydrophobic content in the samples (from 50 to 100% of CLMA). No significant difference between samples with 100% and 70% of CLMA were detected in some cases. Osteoblastic differentiation was followed by assessing the alkaline phosphatase activity of cells, as well as type I collagen and osteocalcin expressions levels until day 21. The three markers were positive at days 14 and 21 when cells were cultured in 100% CLMA substrates which suggests osteoblastic differentiation of mesenchymal stem cells within these scaffolds. On the other hand, when the CLMA content decreases (until 50%), type I collagen and osteocalcin were positive but ALP was negative indicating that the differentiation process is affected by hydrophilic content. We suggest that such system may be useful to extract information on the effect of materials' wettability on the corresponding biological performance in a 3D environment. Such general insights may be relevant in the context of biomaterials selection for tissue engineering strategies. date: 2009-10 date_type: published id_number: 10.1002/jbm.b.31400 uniqueid: glaseprints:2009-76767 published_online: 2009-05-13 issn_online: 1552-4981 scopus_impact: 14 scopus_cluster: 2-s2.0-70049097731 scopus_datestamp: 2013-10-26 09:27:24 wos_impact: 10 wos_cluster: WOS:000269897100032 wos_datestamp: 2013-05-21 22:54:32 full_text_status: none publication: Journal of Biomedical Materials Research Part B: Applied Biomaterials volume: 91B number: 1 pagerange: 277-286 refereed: TRUE issn: 1552-4973 hoa_compliant: 305 hoa_date_pub: 2009-05-13 hoa_exclude: FALSE hoa_gold: FALSE citation: Escobar Ivirico, J.L., Salmerón-Sánchez, M. , Gómez Ribelles, J.L., Monleón Pradas, M., Soria, J.M., Gomes, M.E., Reis, R.L. and Mano, J.F. (2009) Proliferation and differentiation of goat bone marrow stromal cells in 3D scaffolds with tunable hydrophilicity. Journal of Biomedical Materials Research Part B: Applied Biomaterials , 91B(1), pp. 277-286. (doi: 10.1002/jbm.b.31400 )