eprintid: 72783
rev_number: 24
eprint_status: archive
userid: 5672
dir: disk0/00/07/27/83
datestamp: 2012-12-06 15:18:13
lastmod: 2025-03-27 21:48:44
status_changed: 2012-12-06 15:18:13
type: article
metadata_visibility: show
creators_name: Cuchet-Lourenço, D.
creators_name: Boutell, C.
creators_name: Lukashchuk, V.
creators_name: Grant, K.
creators_name: Sykes, A.
creators_name: Murray, J.
creators_name: Orr, A.
creators_name: Everett, R.D.
creators_orcid: 0000-0002-2970-7785
creators_orcid: 0000-0001-8567-0529
title: SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes
ispublished: pub
divisions: 25300000
divisions: 90900000
divisions: 25303100
abstract: Components of promyelocytic leukaemia (PML) nuclear bodies (ND10) are recruited to sites associated with herpes simplex virus type 1 (HSV-1) genomes soon after they enter the nucleus. This cellular response is linked to intrinsic antiviral resistance and is counteracted by viral regulatory protein ICP0. We report that the SUMO interaction motifs of PML, Sp100 and hDaxx are required for recruitment of these repressive proteins to HSV-1 induced foci, which also contain SUMO conjugates and PIAS2β, a SUMO E3 ligase. SUMO modification of PML and elements of its tripartite motif (TRIM) are also required for recruitment in cells lacking endogenous PML. Mutants of PML isoform I and hDaxx that are not recruited to virus induced foci are unable to reproduce the repression of ICP0 null mutant HSV-1 infection mediated by their wild type counterparts. We conclude that recruitment of ND10 components to sites associated with HSV-1 genomes reflects a cellular defence against invading pathogen DNA that is regulated through the SUMO modification pathway.
date: 2011
date_type: published
publisher: Public Library of Science
id_number: 10.1371/journal.ppat.1002123
copyright_holders: Copyright © 2011 The Authors
prior: First published in PLoS ONE 7(7):e100212
repro: Reproduced in accordance with the copyright policy of the publisher
uniqueid: glaseprints:2011-72783
issn_online: 1553-7374
funding_project_code: 65652
funding_award_no: 1
funding_project_name: The role of ubiquitin and ubiquitin-like proteins during viral infection
funding_investigator_name: Chris Boutell
funding_funder_name: Medical Research Council (MRC)
funding_funder_code: MC_UU_12014/5
funding_investigator_dept: MVLS III - CENTRE FOR VIRUS RESEARCH
scopus_impact: 21
scopus_cluster: 2-s2.0-79960942804
scopus_datestamp: 2013-10-26 08:23:08
wos_impact: 20
wos_cluster: WOS:000293339300016
wos_datestamp: 2013-10-25 13:32:58
pubmed_id: 21779164
euro_pubmed_id: 21779164
pmcid: PMC3136452
full_text_status: public
publication: PLoS Pathogens
volume: 7
number: 7
pagerange: e1002123
refereed: TRUE
issn: 1553-7366
hoa_compliant: 305
hoa_date_pub: 2011
hoa_exclude: FALSE
hoa_gold: FALSE
citation:    Cuchet-Lourenço, D. <http://eprints.gla.ac.uk/view/author/4468.html>, Boutell, C. <http://eprints.gla.ac.uk/view/author/3618.html>  <http://orcid.org/0000-0002-2970-7785>, Lukashchuk, V., Grant, K. <http://eprints.gla.ac.uk/view/author/8981.html>, Sykes, A. <http://eprints.gla.ac.uk/view/author/2050.html>  <http://orcid.org/0000-0001-8567-0529>, Murray, J. <http://eprints.gla.ac.uk/view/author/7799.html>, Orr, A. <http://eprints.gla.ac.uk/view/author/2525.html> and Everett, R.D. <http://eprints.gla.ac.uk/view/author/11900.html>  (2011)  SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes.   PLoS Pathogens <https://eprints.gla.ac.uk/view/journal_volume/PLoS_Pathogens.html>, 7(7),  e1002123.   (doi: 10.1371/journal.ppat.1002123 <https://doi.org/10.1371/journal.ppat.1002123>) (PMID:21779164)  (PMCID:PMC3136452)      
document_url: https://eprints.gla.ac.uk/72783/1/72783.pdf