eprintid: 55169
rev_number: 12
eprint_status: archive
userid: 11590
importid: 141
dir: disk0/00/05/51/69
datestamp: 2011-10-20 08:52:07
lastmod: 2022-09-21 17:12:55
status_changed: 2011-10-20 08:52:07
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Kamiri, M.
creators_name: Stift, M
creators_name: Srairi, I.
creators_name: Costantino, G.
creators_name: Moussadik, A.E.
creators_name: Hmyene, A.
creators_name: Bakry, F.
creators_name: Ollitrault, P.
creators_name: Froelicher, Y.
title: Evidence for non-disomic inheritance in a Citrus interspecific tetraploid somatic hybrid between <i>C. reticulata</i> and <i>C. limon</i> using SSR markers and cytogenetic analysis
ispublished: pub
subjects: QK
divisions: 20300000
keywords: Citrus, somatic hybrid, meiotic configuration, intermediate inheritance, molecular analysis
abstract: Artificial tetraploid somatic hybrids have been developed for sterile triploid citrus breeding by sexual hybridization between diploid and tetraploid somatic hybrids. The genetic structure of diploid gametes produced by tetraploid genotypes depends on the mode of chromosome association at meiosis. In order to evaluate tetraploid inheritance in a tetraploid interspecific somatic hybrid between mandarin and lemon, we performed segregation studies using cytogenetic and single sequence repeat molecular markers. Cytogenetic analysis of meiosis in the somatic hybrid revealed 11% tetravalents and 76% bivalents. Inheritance of the tetraploid hybrid was analyzed by genotyping the triploid progeny derived from a cross between a diploid pummelo and the tetraploid somatic hybrid, in order to derive genotypes of the meiospores produced by the tetraploid. A likelihood-based approach was used to distinguish between disomic, tetrasomic, and intermediate inheritance models and to estimate the double reduction rate. In agreement with expectations based the cytogenetic data, marker segregation was largely compatible with tetrasomic and inheritance intermediate between disomic and tetrasomic, with some evidence for preferential pairing of homoeologous chromosomes. This has important implications for the design of breeding programs that involve tetraploid hybrids, and underscores the need to consider inheritance models that are intermediate between disomic and tetrasomic.
date: 2011-08
date_type: published
id_number: 10.1007/s00299-011-1050-x
official_url: http://dx.doi.org/10.1007/s00299-011-1050-x
uniqueid: glaseprints:2011-55169
issn_online: 1432-203X
scopus_impact: 9
scopus_cluster: 2-s2.0-79959696773
scopus_datestamp: 2013-10-26 04:12:06
wos_impact: 7
wos_cluster: WOS:000293795100005
wos_datestamp: 2013-10-25 07:05:46
legacy_divisions: L25000000
full_text_status: none
publication: Plant Cell Reports
volume: 30
number: 8
pagerange: 1415-1425
refereed: TRUE
issn: 0721-7714
related_url_url: http://www.ncbi.nlm.nih.gov/pubmed/21409551
related_url_type: pubmed
hoa_compliant: 305
hoa_date_pub: 2011-08
hoa_exclude: FALSE
hoa_gold: FALSE
citation:    Kamiri, M., Stift, M <https://eprints.gla.ac.uk/view/author/10075.html>, Srairi, I., Costantino, G., Moussadik, A.E., Hmyene, A., Bakry, F., Ollitrault, P. and Froelicher, Y.  (2011)  Evidence for non-disomic inheritance in a Citrus interspecific tetraploid somatic hybrid between C. reticulata and C. limon using SSR markers and cytogenetic analysis.   Plant Cell Reports <https://eprints.gla.ac.uk/view/journal_volume/Plant_Cell_Reports.html>, 30(8),  pp. 1415-1425.   (doi: 10.1007/s00299-011-1050-x <https://doi.org/10.1007/s00299-011-1050-x>)