eprintid: 27188
rev_number: 8
eprint_status: archive
userid: 14001
dir: disk0/00/02/71/88
datestamp: 2010-04-28 14:39:53
lastmod: 2016-08-25 11:17:45
status_changed: 2010-04-28 14:39:53
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Hodgkinson, JE
creators_name: Love, S
creators_name: Lichtenfels, JR
creators_name: Palfreman, S
creators_name: Ramsey, YH
creators_name: Matthews, JB
title: Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses
ispublished: pub
divisions: 20300000
abstract: Here, we report evaluation of five oligoprobes designed from intergenic spacer (IGS) region sequences for identification of cyathostomin species. Oligoprobes were designed for identification of Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicostephanus longibursatus, Cylicostephanus goldi and a fifth probe designed to identify all members of this tribe. PCR amplification of IGS DNA from 16 cyathostomin species allowed sequence comparison and identification of four putative species-specific probes. Southern blotting of amplified products from 16 species showed that all probes were species-specific. The fifth probe recognised all 16 cyathostomin species but did not bind to members of the genus Strongylus. Furthermore, these probes were used to identify individual infective L3, eggs and L4 indicating that they will be invaluable to furthering the study of the epidemiology and pathogenesis of these important equine nematodes.
date: 2001-02
date_type: published
publisher: Elsevier
id_number: 0.1016/S0020-7519(00)00161-2
uniqueid: glaseprints:2001-27188
thomson_ut: 167513600009
published_online: 2001-02-28
issn_online: 1879-0135
scopus_impact: 23
scopus_cluster: 2-s2.0-0035118933
scopus_datestamp: 2013-10-25 21:35:04
wos_impact: 23
wos_cluster: WOS:000167513600009
wos_datestamp: 2013-10-24 22:52:20
full_text_status: none
publication: International Journal for Parasitology
volume: 31
number: 2
pagerange: 197-204
refereed: TRUE
issn: 0020-7519
hoa_compliant: 304
hoa_date_pub: 2001-02-28
citation:    Hodgkinson, JE, Love, S <https://eprints.gla.ac.uk/view/author/11333.html>, Lichtenfels, JR, Palfreman, S, Ramsey, YH and Matthews, JB  (2001)  Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses.   International Journal for Parasitology <https://eprints.gla.ac.uk/view/journal_volume/International_Journal_for_Parasitology.html>, 31(2),  pp. 197-204.   (doi: 0.1016/S0020-7519(00)00161-2)