TY  - JOUR
N1  - L.A.D. was supported by a scholarship from Jeddah University and the Saudi Arabian Government. The work was also supported by grants from EPSRC (EP/K034898/1) and MRC (MR/S010343/1).
SN  - 0142-9612
VL  - 280
N2  - Post-operative infection is a major complication in patients recovering from orthopaedic surgery. As such, there is a clinical need to develop biomaterials for use in regenerative surgery that can promote mesenchymal stem cell (MSC) osteospecific differentiation and that can prevent infection caused by biofilm-forming pathogens. Nanotopographical approaches to pathogen control are being identified, including in orthopaedic materials such as titanium and its alloys. These topographies use high aspect ratio nanospikes or nanowires to prevent bacterial adhesion but these features also significantly reduce MSC adhesion and activity. Here, we use a poly (ethyl acrylate) (PEA) polymer coating on titanium nanowires to spontaneously organise fibronectin (FN) and to deliver bone morphogenetic protein 2 (BMP2) to enhance MSC adhesion and osteospecific signalling. Using a novel MSC?Pseudomonas aeruginosa co-culture, we show that the coated nanotopographies protect MSCs from cytotoxic quorum sensing and signalling molecules, enhance MSC adhesion and osteoblast differentiation and reduce biofilm formation. We conclude that the PEA polymer-coated nanotopography can both support MSCs and prevent pathogens from adhering to a biomaterial surface, thus protecting from biofilm formation and bacterial infection, and supporting osteogenic repair.
AV  - public
Y1  - 2022/01//
JF  - Biomaterials
A1  - Damiati, Laila A.
A1  - Tsimbouri, Monica P.
A1  - Llopis Hernandez, Virginia
A1  - Jayawarna, Vineetha
A1  - Ginty, Mark
A1  - Childs, Peter
A1  - Xiao, Yinbo
A1  - Burgess, Karl
A1  - Wells, Julia
A1  - Sprott, Mark R.
A1  - Meek, R.M. Dominic
A1  - Li, Peifeng
A1  - Oreffo, Richard O.C.
A1  - Nobbs, Angela
A1  - Ramage, Gordon
A1  - Su, Bo
A1  - Salmeron-Sanchez, Manuel
A1  - Dalby, Matthew J.
UR  - https://eprints.gla.ac.uk/258932/
ID  - enlighten258932
PB  - Elsevier
TI  - Materials-driven fibronectin assembly on nanoscale topography enhances mesenchymal stem cell adhesion, protecting cells from bacterial virulence factors and preventing biofilm formation
ER  -