eprintid: 22671
rev_number: 16
eprint_status: archive
userid: 14001
dir: disk0/00/02/26/71
datestamp: 2009-12-19 16:06:11
lastmod: 2025-05-01 13:52:05
status_changed: 2009-12-19 16:06:11
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Mallon, M.E.
creators_name: MacLeod, A.
creators_name: Wastling, J.M.
creators_name: Smith, H.
creators_name: Tait, A.
creators_orcid: 0000-0002-0150-5049
title: Multilocus genotyping of Cryptosporidium parvum Type 2: population genetics and sub-structuring.
ispublished: pub
subjects: QH426
divisions: 20000000
divisions: 20300000
abstract: Cryptosporidium parvum is an intracellular protozoan parasite that infects the gastrointestinal tract of humans and other mammals. It has significant economic importance as a pathogen of livestock and, as there is no effective treatment or vaccine available, understanding transmission routes and identifying sources of infection is key to preventing future outbreaks and controlling this disease. In this study we have determined the multilocus genotype (MLG) of 240 C. parvum Type 2 (bovine) isolates using a combination of seven micro- and minisatellite markers. These isolates were collected over a period of 19 months and are from three different geographical locations within Scotland and three different host species. The results of this study have enabled us to address questions concerning C. parvum population genetics in relation to host, temporal and geographical sub-structuring. We identified 48 multilocus genotypes within the Type 2 C. parvum isolates and found no evidence to support geographic or temporal sub-structuring of the populations. However host sub-structuring was identified within the human Type 2 population highlighting the potential use of such a typing system in understanding the epidemiology of this parasite in addition to raising interesting questions with regard to its population genetic structure. We also isolated two C. parvum ‘monkey type’ isolates from two separate human cases indicating that this genotype is not restricted to monkey hosts with the multilocus genotypes of these isolates distinguishing them from all other isolates.
date: 2003-09
date_type: published
publisher: Elsevier
id_number: 10.1016/S1567-1348(03)00089-3
uniqueid: glaseprints:2003-22671
scopus_impact: 94
scopus_cluster: 2-s2.0-0141605224
scopus_datestamp: 2013-10-25 20:33:35
legacy_divisions: L25000000
legacy_divisions: L20300000
full_text_status: none
publication: Infection, Genetics and Evolution
volume: 3
number: 3
pagerange: 207-218
refereed: TRUE
issn: 1567-1348
hoa_compliant: 305
hoa_date_acc: 2003-06-05
hoa_date_pub: 2003-09-06
hoa_exclude: FALSE
hoa_gold: FALSE
citation:    Mallon, M.E., MacLeod, A. <https://eprints.gla.ac.uk/view/author/2249.html>, Wastling, J.M., Smith, H. <https://eprints.gla.ac.uk/view/author/6446.html> and Tait, A. <https://eprints.gla.ac.uk/view/author/2592.html>  (2003)  Multilocus genotyping of Cryptosporidium parvum Type 2: population genetics and sub-structuring.   Infection, Genetics and Evolution <https://eprints.gla.ac.uk/view/journal_volume/Infection,_Genetics_and_Evolution.html>, 3(3),  pp. 207-218.   (doi: 10.1016/S1567-1348(03)00089-3 <https://doi.org/10.1016/S1567-1348(03)00089-3>)