relation: https://eprints.gla.ac.uk/20120/ title: Characterisation of bacterial communities associated with toxic and non-toxic dinoflagellates: <i>Alexandrium</i> spp. and <i>Scrippsiella trochoidea</i> creator: Hold, Georgina L. creator: Smith, Elizabeth A. creator: Rappe, Michael S. creator: Maas, Elizabeth W creator: Moore, Edward R.B. creator: Stroempl, Carsten creator: Stephen, John R. creator: Prosser, James I. creator: Birkbeck, T. Harry creator: Gallacher, Susan description: Several dinoflagellate species have been shown to produce potent neurotoxins known as paralytic shellfish toxins. Evidence is also accumulating that marine bacteria associated with dinoflagellates play a role in the accumulation of paralytic shellfish toxins. In this study, the diversity of bacteria in cultures of both toxic and non-toxic dinoflagellates, <i>Alexandrium</i> spp. and <i>Scrippsiella trochoidea</i>, were compared using colony morphology, restriction fragment length polymorphisms, denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes and, ultimately, sequence determination of the 16S rRNA genes. The results suggest that a number of different bacterial species are associated with dinoflagellates, some of which are common to each of the dinoflagellate cultures examined, whereas others appear to be unique to a particular dinoflagellate. The phylogenetic diversity of the bacteria observed was limited to two bacterial phyla, the Proteobacteria and the Cytophaga-Flavobacter-Bacteroides (CFB). Although phylum level diversity was limited, many distinct phylogenetic clades were recovered, including members of both the α- and γ-subclasses of the Proteobacteria. Additionally, several of the bacterial phylotypes isolated were not closely related to any published bacterial species but, rather, were identical to isolates characterised from <i>Alexandrium</i> cultures 4 years earlier. Finally, many of the bacteria isolated from the dinoflagellate cultures were related to microorganisms with known surface-associated life histories (e.g. the CFB phylum, <i>Hyphomonas, Caulobacter</i> and some members of the <i>Roseobacter</i> clade including <i>Ruegeria algicola</i>). publisher: Wiley date: 2001-10 type: Articles type: PeerReviewed identifier: Hold, G. L., Smith, E. A., Rappe, M. S., Maas, E. W., Moore, E. R.B., Stroempl, C., Stephen, J. R., Prosser, J. I., Birkbeck, T. H. <http://eprints.gla.ac.uk/view/author/12417.html> and Gallacher, S. (2001) Characterisation of bacterial communities associated with toxic and non-toxic dinoflagellates: Alexandrium spp. and Scrippsiella trochoidea. FEMS Microbiology Ecology <https://eprints.gla.ac.uk/view/journal_volume/FEMS_Microbiology_Ecology.html>, 37(2), pp. 161-173. (doi: 10.1111/j.1574-6941.2001.tb00864.x <https://doi.org/10.1111/j.1574-6941.2001.tb00864.x>) relation: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00864.x relation: 10.1111/j.1574-6941.2001.tb00864.x identifier: 10.1111/j.1574-6941.2001.tb00864.x