RT Journal Article
SR 00
ID 10.1515/bc.1999.036
A1 Bischof, J.
A1 Vietor, I.
A1 Cotten, M.
A1 Huber, L.A.
T1 Transient transfection of mammary epithelial cells with a PEI/DNA/adenovirus system
JF Biological Chemistry
YR 1999
FD 1999-02
VO 380
IS 2
SP 269
OP 273
AB Studies on epithelial cells often require the transient expression of exogenous proteins in polarized epithelial cells. However, the major limitation of this approach has been the difficulty of obtaining transient gene expression in polarized epithelial cell cultures. We report here on the application of a polyethylenimine (PEI)/DNA/adenovirus system for efficient transient gene expression in mammary epithelial cells. Based on luciferase assay and FACScan analysis the PEI/DNA/adenovirus system is shown to be an effective and simple method for transfecting epithelial cells.
PB De Gruyter
SN 1431-6730
LK https://eprints.gla.ac.uk/195218/