Surgery-induced neuroinflammatory transcriptional programs in medial prefrontal cortex of mice during early phase of perioperative neurocognitive disorders

View article
PeerJ

Main article text

 

Introduction

Materials and methods

Animals and experimental design

Surgery of tibial fracture

Open-field test

Fear conditioning test

Training

Testing

RNA-seq analysis

qRT–PCR

Measurement of cytokine levels

Statistical analysis

Results

Cognitive decline after surgery

Transcriptome profile in the mPFC

GO enrichment analysis

KEGG pathway analysis

qRT–PCR validation of RNA-seq results

Levels of inflammatory cytokines

Discussion

Conclusions

Supplemental Information

Primers used in the study for qRT-PCR.

DOI: 10.7717/peerj.18664/supp-1

All differentially expressed genes.

DOI: 10.7717/peerj.18664/supp-2

Functional properties of genes selected for qRT-PCR analysis.

DOI: 10.7717/peerj.18664/supp-3

ARRIVE 2.0 Checklist.

DOI: 10.7717/peerj.18664/supp-4

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Xiaodong Tang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Xuwu Xiang performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Yang Yu performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Shuyuan Huang performed the experiments, prepared figures and/or tables, and approved the final draft.

Caifei Pan performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Shuyuan Gan performed the experiments, prepared figures and/or tables, and approved the final draft.

Yongxing Yao conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Animal Care Committee of the First Affiliated Hospital at Zhejiang University School of Medicine (Hangzhou, China) approved this study.

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

The raw sequence files for mRNA sequencing are deposited in the NCBI Sequence Read Archive database: PRJNA1077729.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplemental Files.

Funding

The research was supported by the Zhejiang Provincial Natural Science Foundation of China (LQ23H090013 and LBY22H270007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

406 Visitors 384 Views 18 Downloads