Preconceptual Zika virus asymptomatic infection protects against secondary prenatal infection
Fig 4
Primary ZIKV asymptomatic infection protects against re-infection during pregnancy.
(A) Schematic illustrating when mating is initiated and secondary ZIKV infection occurs amongst mice with prior asymptomatic primary infection compared with naive control mice. ZIKV genome copies in each maternal tissue, each individual concepti (placenta, decidua, and fetal tissue), or averaged among individual concepti in each litter day 3 after ZIKV re-infection at midgestation (E11.5). (B) IgG antibody titers (A450) with ZIKV ENV specificity in the serum of mice described in panel A (C) Percent reduction in ZIKV plaques after pre-incubation with each dilution of the serum each group of mice described in panel A. (D) Scatterplot comparing ZIKV genomic copies and ENV IgG antibody levels (A450) in clarified fetal homogenates amongst individual concepti described in panel A. (E) Number of ZIKV plaques after pre-incubation with clarified homogenates of individual concepti described in panel A. Each point depicts the data from an individual mouse that is representative of at least three independent experiments each with similar results. Bar, mean ± one standard error; LOD, limits of detection; * p < 0.05; ** p < 0.01; *** p < 0.001.
doi: https://doi.org/10.1371/journal.ppat.1006684.g004