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Analysis of the T Cell Response to Zika Virus and Identification of a Novel CD8⁺ T Cell Epitope in Immunocompetent Mice

Fig 8

CD8⁺ T cells respond to an epitope within the ZIKV Envelope protein.

(A) Representative plots of IFN-γ production from antigen-experienced CD8α^loCD11a^hi CD8⁺ T cells (top row) and naïve CD8α^hiCD11a^lo CD8⁺ T cells (bottom row) from the spleens of ZIKV-infected mice 7 dpi. Total splenocytes were incubated for 5.5 h at 37°C with media alone or 200nM of the indicated peptide in the presence of Brefeldin A. (B) Representative plots of IFN-γ production from antigen-experienced CD8α^loCD11a^hi CD8⁺ T cells (top row) and naïve CD8α^hiCD11a^lo CD8⁺ T cells (bottom row) from the spleens LCMV-infected mice 7 dpi. Total splenocytes were incubated for 5.5 h at 37°C with media alone or 200nM of the indicated peptide in the presence of Brefeldin A. (C-D) Percentage of IFN-γ⁺ antigen-experienced CD8α^loCD11a^hi or naïve CD8α^hiCD11a^lo CD8⁺ T cells from mock-, UV-inactivated ZIKV-, ZIKV- or LCMV-infected mice 7 dpi after restimulation with media alone or 200nM of GP_33-41 (LCMV peptide) (C) or Env_294-302 (ZIKV peptide) (D) for 5.5 h at 37°C in the presence of Brefeldin A. Error bars represent mean ± SEM. Data are pooled from two independent experiments, n = 3 mice per group per experiment. Data in (C and D) were analyzed with a two-tailed, paired Student’s t test. ****p<0.0001.

doi: https://doi.org/10.1371/journal.ppat.1006184.g008