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Analysis of the T Cell Response to Zika Virus and Identification of a Novel CD8⁺ T Cell Epitope in Immunocompetent Mice

Fig 7

Antigen-experienced CD8⁺ T cells present with an activated phenotype at the peak of the T cell response.

(A) Representative plot of CD127 and KLRG1 expression on CD8α^loCD11a^hi CD8⁺ T cells from spleens of mock-infected mice 7 dpi. Percent (B) and number (C) of CD127^loKLRG1^hi short-lived effector cells (SLECs) and CD127^hiKLRG1^lo memory precursor effector cells (MPECs) on CD8α^loCD11a^hi CD8⁺ T cells from mock-infected mice. (D) Representative plot of CD127 and KLRG1 expression on CD8α^loCD11a^hi CD8⁺ T cells from spleens of ZIKV-infected mice 7 dpi. Percent (E) and number (F) of SLECs and MPECs on CD8α^loCD11a^hi CD8⁺ T cells from ZIKV-infected mice. Representative histogram (G), percentage (H), and number (I) of CD62L⁺ and CD62L^- CD8α^loCD11a^hi CD8⁺ T cells from spleens of mock- (shaded histogram) and ZIKV-infected (open histogram) mice 7 dpi. Numbers on histogram indicate percentage of CD62L^- (left gate) and CD62L⁺ (right gate) cells from ZIKV-infected sample. (J) Representative histograms of granzyme B expression in CD8α^loCD11a^hi CD8⁺ T cells from spleens of mock- (left) and ZIKV-infected (right) mice 7 dpi. Shaded histograms represent isotype control. (K) gMFI of granzyme B expression. Error bars represent mean ± SEM. Data are pooled from two independent experiments, n = 3–5 mice per group per experiment. Data in (H, I and K) were analyzed with a two-tailed, unpaired Student’s t test. ***p<0.0005; ****p<0.0001.

doi: https://doi.org/10.1371/journal.ppat.1006184.g007