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Analysis of the T Cell Response to Zika Virus and Identification of a Novel CD8⁺ T Cell Epitope in Immunocompetent Mice

Fig 6

ZIKV infection induces a prototypical effector CD8⁺ T cell response in immunocompetent mice.

Representative plots of IFN-γ (A), TNF-α (B), and IL-2 (C) production from splenic CD8α^loCD11a^hi CD8⁺ T cells from ZIKV-infected mice 7 dpi. Total splenocytes were incubated in media alone in the presence of Brefeldin A (top) or stimulated with PMA and ionomycin in the presence of Brefeldin A for 3 h at 37°C (bottom). Percentage (D), number (E), and gMFI (F) of cytokine-producing CD8α^loCD11a^hi CD8⁺ T cells from the spleens of ZIKV-infected mice 7 dpi. (G) Representative histograms of T-bet expression in splenic CD8α^loCD11a^hi CD8⁺ T cells from mock- and ZIKV-infected mice 7 dpi. Shaded histograms represent isotype control. Percentage (H) and gMFI (I) of T-bet expression in splenic CD8α^loCD11a^hi CD8⁺ T cells from mock- and ZIKV-infected mice 7 dpi. Error bars represent mean ± SEM. Data are pooled from two independent experiments, n = 3–5 mice per group per experiment. Data in (H and I) were analyzed with a two-tailed, unpaired Student’s t test. ****p<0.0001.

doi: https://doi.org/10.1371/journal.ppat.1006184.g006