Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor
Figure 2
Pyocin L1 binds strongly to CPA from P. aeruginosa PAO1.
(A) ITC binding isotherm of pyocin L1 (150 µM) titrated into isolated LPS-derived polysaccharide (1 mg ml−1) from wild-type P. aeruginosa PAO1. Strong, saturable heats were observed indicative of a strong interaction. Curve fitted with a single binding site model. (B) ITC isotherm of pyocin L1 (150 µM) titrated into isolated LPS-derived polysaccharide (1 mg ml−1) from PAO1 wzt. No saturable binding isotherm was observed.
doi: https://doi.org/10.1371/journal.ppat.1003898.g002