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Listeria monocytogenes Cytoplasmic Entry Induces Fetal Wastage by Disrupting Maternal Foxp3⁺ Regulatory T Cell-Sustained Fetal Tolerance

Figure 6

Listeria monocytogenes cytoplasmic entry dampens maternal Foxp3⁺ regulatory CD4 T cell suppressive potency.

(A) Percent GFP⁺ or Foxp3⁺ among CD4 cells in pregnant mice midgestation after infection with LmΔactA (10⁷ CFUs) or LmΔLLOΔPLC (10⁸ CFUs). (B) Representative plots demonstrating proliferation (CFSE dilution) among responder (Tresp) CD90.1⁺ CD8 cells after co-culture with each ratio of GFP⁺ Tregs isolated from LmΔactA (10⁷ CFUs) or LmΔLLOΔPLC (10⁸ CFUs) infected mice, and stimulation with anti-CD3 antibody (black line), compared with no Treg (gray filled) or no stimulation (black filled) controls (top). Relative suppression of responder cell proliferation (CFSE dilution) after co-culture with GFP⁺ Tregs for cells from mice described above normalized to suppression by GFP⁺ cells from uninfected controls at a 1∶1 Treg∶Tresp ratio (bottom). These data reflect six to eight mice per group representative of three independent experiments each with similar results.

doi: https://doi.org/10.1371/journal.ppat.1002873.g006