Listeria monocytogenes Cytoplasmic Entry Induces Fetal Wastage by Disrupting Maternal Foxp3+ Regulatory T Cell-Sustained Fetal Tolerance
Figure 3
Listeria monocytogenes infection during pregnancy dampens maternal Foxp3+ regulatory CD4 T cell suppressive potency.
(A) Percent GFP+ or Foxp3+ among CD4 cells in virgin, pregnant mice midgestation without infection, or three days after infection with 104 WT Lm. (B) Representative plots demonstrating proliferation (CFSE dilution) among responder (Tresp) CD90.1+ CD8 cells after co-culture with each ratio of GFP+ Tregs isolated from Lm-infected mice and stimulation with anti-CD3 antibody (black line), compared with no Treg (gray filled) or no stimulation (black filled) controls (top). Relative suppression of responder cell proliferation (CFSE dilution) after co-culture with GFP+ Tregs for the mice described above normalized to suppression by GFP+ cells from uninfected controls at a 1∶1 Treg∶Tresp ratio (bottom). These data reflect six to eight mice per group representative of three independent experiments each with similar results.
doi: https://doi.org/10.1371/journal.ppat.1002873.g003