SUMO Pathway Dependent Recruitment of Cellular Repressors to Herpes Simplex Virus Type 1 Genomes
Figure 8
Recruitment of hDaxx to sites associated with HSV-1 genomes is dependent on its SIM.
A. Map of hDaxx and its major features (PAH1 and PAH2 – paired amphipathic helices 1 and 2; D/E – aspartic and glutamic acid rich region; S/P/T – serine, proline and threonine rich region). The position and sequence of the SIM and the changes in the mSIM mutant are indicated. B. Western blot of control and hDaxx depleted cells, and depleted cells reconstituted with the wt and mSIM mutant EYFP-hDaxx, using anti-hDaxx (upper panel) and anti-EGFP (lower panel) antibodies. C. Typical images of wt and mSIM mutant EYFP-hDaxx (green) in uninfected hDaxx depleted cells co-stained for endogenous PML (red) (upper panels), and assays of their recruitment to sites associated with HSV-1 genomes (ICP4; red) in cells at the edges of ICP0 null mutant (ΔICP0) plaques (lower panels). Scale bars indicate 5 µm.
doi: https://doi.org/10.1371/journal.ppat.1002123.g008