Histological Assessment of PAXgene Tissue Fixation and Stabilization Reagents
Figure 1
Morphology of various normal tissues.
Figure 1a shows H&E stains in tissues fixed for 3 hours in PAXgene (left column), 24 hours in PAXgene (centre column) and for comparative purposes routine fixation in formalin for 24 hours (right column). In liver PAXgene fixation scores better on contrast. Striated muscle shows more detail after PAXgene fixation. Thyroid tissue scores better in all scored details. And adrenal gland tissue predominantly scores better in membrane detail. (400× original magnification). Figure 1b shows H&E stains in tissues fixed for 3 hours in PAXgene (left column), 24 hours in PAXgene (center column) and for comparative purposes routine fixation in formalin for 24 hours (right column). Lung tissue appears swollen. In gastric tissue the cell differentiation is harder to distinguish. In colon the distinctive granules in Paneth cells are less easily detectable after PAXgene fixation and in prostate the contrast of the epithelium is lower in PFPE tissue compared to FFPE tissue. (Lung and prostate 400×; gastric and colon tissue 200× original magnification).
doi: https://doi.org/10.1371/journal.pone.0027704.g001