Simone Zanetti

Simone Zanetti

Bellinzona, Tessin, Schweiz
700 Follower:innen 500+ Kontakte

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PhD Scientist and Supervisor with >12 years experience in life science. I have…

Aktivitäten

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Berufserfahrung

  • CSL Behring Grafik

    CSL Behring

    Global

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    Berne, Switzerland

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    Kankakee, Illinois, United States

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    Bern Area, Switzerland

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    Bern Area, Switzerland

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    Epalinges, Vaud, Switzerland

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    Fribourg, Fribourg, Switzerland

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    Fribourg, Fribourg, Switzerland

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    Fribourg, Fribourg, Switzerland

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    Bellinzona, Ticino, Switzerland

Ausbildung

Veröffentlichungen

  • Casein: From Wine Fining to Allergenic Food Regulations

    Caseins: Properties, Functions and Health Implications (NOVA Science Publisher)

    Since the XVIIIth century, the use of fining agents for wine production has been a standard procedure. Nowadays, casein is one of the most commonly used processing aids for the clarification of both white and red wines. Casein is extracted from milk. As allergy to cow's milk also concerns adult consumers, researchers have focused on the possibility that wine consumption could elicit an allergic reaction to the residual casein-based fining agent. In this chapter we summarize the published…

    Since the XVIIIth century, the use of fining agents for wine production has been a standard procedure. Nowadays, casein is one of the most commonly used processing aids for the clarification of both white and red wines. Casein is extracted from milk. As allergy to cow's milk also concerns adult consumers, researchers have focused on the possibility that wine consumption could elicit an allergic reaction to the residual casein-based fining agent. In this chapter we summarize the published clinical studies on this topic and conclude that an adverse reaction due to the accidental ingestion of casein by wine consumption is rather unlikely.
    Winemaking procedures can differ from one winery to another, but the doses of fining agents are recommended by the international organization of vine and wine. To evaluate the allergic risk for sensitive consumers, the possibility to find residual casein in commercial wines has been investigated. Results are summarized in this review. To date, the presence of residual casein in wine cannot be excluded. We therefore surveyed published works on commercial and experimental wines. Based on these studies, we conclude that a bentonite treatment and a final filtration are essential steps to obtain a casein-free final product. Of course, the possibility to detect trace amounts of casein lies within detection limits of the analytical method. Therefore, we also discuss and compare such procedures to support readers in choosing the most adapted method.
    The criteria to evaluate the risk of an adverse reaction due to residual allergens in wine strongly differ from one nation to the other. Therefore, we compare and discuss food regulations of different nations with an important winemaking tradition.

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  • Detection of Traces of Ovalbumin and Casein in White and Red Wines by Quantitative Western Blotting

    Journal of Food Science

    Fining of wine with agents containing cow's milk or hen's egg white is a common and traditional procedure. In light of increasing food allergies all over the world, the presence of fining residues has been subject of intense debate. Switzerland does not make exception, and since 2009 the Federal Department of Home Affairs has modified its food regulations stating that the labels must show if traces of fining agents are present. Nevertheless, the application of this regulation is not based on an…

    Fining of wine with agents containing cow's milk or hen's egg white is a common and traditional procedure. In light of increasing food allergies all over the world, the presence of fining residues has been subject of intense debate. Switzerland does not make exception, and since 2009 the Federal Department of Home Affairs has modified its food regulations stating that the labels must show if traces of fining agents are present. Nevertheless, the application of this regulation is not based on an official analytical method. In this study we show that immunoblotting is an efficient technique to detect and quantify ovalbumin and casein residues in bottled wine. We showed that final filtration is an essential step to remove finings in red wine, and that overfining of white wine may result in fining residues in finished products. Finally, for the first time in Switzerland, 22 samples were taken by food safety inspectors and officially analyzed for the regional food control authority of the Canton of Vaud. These samples were allergen free, but a larger study is currently planned in collaboration with other regional authorities of Switzerland to complete these results and make a complete picture of the Swiss wine production.

    Andere Autor:innen
    • Philippe Meyer
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  • Steak tartare: sale histoire de cru

    RTS - A Bon Entendeur

    Les bactéries se démultiplient dans la viande coupée en petits morceaux. Le tartare qu’on vous sert en restaurant vous fait-il courir des risques, un peu ou pas du tout? C’est ce qu’ABE a voulu vérifier par des prélèvements très codifiés.

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  • Verdure préemballée: Croquez, c’est sans danger !

    Fédération romande des consommateurs

    A la suite du scandale de la bactérie
    tueuse ECEH en 2011, l’Europe et
    la Suisse ont su tirer de bonnes
    leçons. Des lots de graines germées
    et de salades en sachet ont été
    testés: aucun n’est contaminé.

    Andere Autor:innen
    • Huma Khamis
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  • Sex Determination in the Caenorhabditis elegans Germline

    Advances in Experimental Medicine and Biology; Springer

    How is sex determined? In the animal kingdom, there are diverse sets of mechanisms for determining organismal sex, with the predominant ones being chromosomally based, either a dominant-acting sex chromosome or the ratio of the number of X chromosome to autosomes, which lead to oocyte-producing females and sperm-producing males. The resulting germline sexual phenotype is often the logical consequence of somatic sex determination. In this respect however, the Caenorhabditis elegans hermaphrodite…

    How is sex determined? In the animal kingdom, there are diverse sets of mechanisms for determining organismal sex, with the predominant ones being chromosomally based, either a dominant-acting sex chromosome or the ratio of the number of X chromosome to autosomes, which lead to oocyte-producing females and sperm-producing males. The resulting germline sexual phenotype is often the logical consequence of somatic sex determination. In this respect however, the Caenorhabditis elegans hermaphrodite is different from mammals and Drosophila. In fact in the C. elegans hermaphrodite germline, male gametes are transiently produced in a female body during larval development. To override chromosomal signals, sex determination of germ cells strongly depends on post-transcriptional regulation. A pivotal role for male gamete production (spermatogenesis) is played by the fem-3 mRNA, which is controlled through FBF and other RNA-binding proteins or splicing factors. Thanks to its powerful genetics, transparent body, small size, and the ability to make sperm and oocytes within one individual, C. elegans represents an excellent system to investigate cellular differentiation and post-transcriptional control.

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  • The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

    RNA Journal

    Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 3' UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that…

    Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 3' UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that control fem-3. Here, we investigate the steady-state levels of the fem-3 transcript and the expression pattern of its protein product. We show that FEM-3 is exclusively present in germ cells that are committed to spermatogenesis. We found that in fem-3(gf)/+ heterozygotes, mutant fem-3 gain-of-function transcripts are more abundant than their wild-type counterpart. Furthermore, we show that the penetrance of the fem-3(gf) allele correlates with inefficient FBF binding and extended poly(A) tail size of fem-3 mRNAs. Finally, we show that wild-type and gain-of-function mutated fem-3 mRNAs associate equally well with polyribosomes. We propose that the fem-3 mRNA is regulated through stabilization rather than through translatability.

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  • Un été au Rwanda

    Unireflets; University of Fribourg

    Comment faire rimer voyage et partage ? Simone Zanetti et Cristina Martignoli, étudiants en biologie et pédagogie curative, ont trouvé la réponse au Rwanda.

    Andere Autor:innen
    • Cristina Martignoli
    • Farida Khali
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  • Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection

    Developmental Biology, Elsevier

    In Caenorhabditis elegans, germ cells develop as spermatids in the larva and as oocytes in the adult. Such fundamentally different gametes are produced through a fine-tuned balance between feminizing and masculinizing genes. For example, the switch to oogenesis requires repression of the fem-3 mRNA through the mog genes. Here we report on the cloning and characterization of the sex determination gene mog-2. MOG-2 is the worm homolog of spliceosomal protein U2A'. We found that MOG-2 is expressed…

    In Caenorhabditis elegans, germ cells develop as spermatids in the larva and as oocytes in the adult. Such fundamentally different gametes are produced through a fine-tuned balance between feminizing and masculinizing genes. For example, the switch to oogenesis requires repression of the fem-3 mRNA through the mog genes. Here we report on the cloning and characterization of the sex determination gene mog-2. MOG-2 is the worm homolog of spliceosomal protein U2A'. We found that MOG-2 is expressed in most nuclei of somatic and germ cells. In addition to its role in sex determination, mog-2 is required for meiosis. Moreover, MOG-2 binds to U2B″/RNP-3 in the absence of RNA. We also show that MOG-2 associates with the U2 snRNA in the absence of RNP-3. Therefore, we propose that MOG-2 is a bona fide component of the U2 snRNP. Albeit not being required for general pre-mRNA splicing, MOG-2 increases the splicing efficiency to a cryptic splice site that is located at the 5' end of the exon.

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  • The C. elegans sex determination protein MOG-3 functions in meiosis and binds to the CSL co-repressor CIR-1

    Developmental Biology, Elsevier

    In the germ line of the Caenorhabditis elegans hermaphrodite, nuclei either proliferate through mitosis or initiate meiosis, finally differentiating as spermatids or oocytes. The production of oocytes requires repression of the fem-3 mRNA by cytoplasmic FBF and nuclear MOG proteins. Here we report the identification of the sex determining gene mog-3 and show that in addition to its role in gamete sex determination, it is necessary for meiosis by acting downstream of GLP-1/Notch. Furthermore, we…

    In the germ line of the Caenorhabditis elegans hermaphrodite, nuclei either proliferate through mitosis or initiate meiosis, finally differentiating as spermatids or oocytes. The production of oocytes requires repression of the fem-3 mRNA by cytoplasmic FBF and nuclear MOG proteins. Here we report the identification of the sex determining gene mog-3 and show that in addition to its role in gamete sex determination, it is necessary for meiosis by acting downstream of GLP-1/Notch. Furthermore, we found that MOG-3 binds both to the nuclear proteins MEP-1 and CIR-1. MEP-1 is necessary for oocyte production and somatic differentiation, while the mammalian CIR-1 homolog counters Notch signaling. We propose that MOG-3, MEP-1 and CIR-1 associate in a nuclear complex which regulates different aspects of germ cell development. While FBF triggers the sperm/oocyte switch by directly repressing the fem-3 mRNA in the cytoplasm, the MOG proteins play a more indirect role in the nucleus, perhaps by acting as epigenetic regulators or by controlling precise splicing events.

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Auszeichnungen/Preise

  • Prize of the Faculty of Science 2012 in Life Sciences

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Sprachen

  • English

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  • Italian

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  • French

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  • German

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