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Cellosaurus IM-9 (CVCL_1305)

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Cell line name IM-9
Synonyms IM 9; IM9; GM04680
Accession CVCL_1305
Resource Identification Initiative To cite this cell line use: IM-9 (RRID:CVCL_1305)
Comments Problematic cell line: Misclassified. Originally thought to be a myeloma cell line but is a B-lymphoblastoid cell line (PubMed=10516762).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: ~45 hours (DSMZ=ACC-117); ~1 day (Note=Lot 09172008), ~23 hours (Note=Lot 03302009) (JCRB=JCRB0024).
Microsatellite instability: Stable (MSS) (Sanger).
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (Cosmic-CLP=753563; DepMap=ACH-002247).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.41
East Asian, North0
East Asian, South0
South Asian0
European, North68.16
European, South31.43
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B6CV (IM-9-P)
Sex of cell Female
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): ATCC=CCL-159; CCRID=1102HUM-NIFDC00064; Cosmic-CLP=753563; DSMZ=ACC-117; JCRB=JCRB0024; KCLB=10159

Markers:
AmelogeninX
CSF1PO10,11
D2S133824,26
D3S135814,18
D5S81813
D7S82011,12
D8S117912,13
D13S3179,11
D16S5399,13
D18S5114,17
D19S43313,14
D21S1130,30.2
FGA20,23
Penta D9,11
Penta E13,15
TH016,9.3
TPOX11
vWA14,17

Run an STR similarity search on this cell line
Publications

PubMed=2836165; DOI=10.1210/endo-122-6-2508
Gabriel E. DiMattia, Birgit Gellersen, Heinz G. Bohnet, Henry George Friesen;
A human B-lymphoblastoid cell line produces prolactin.
Endocrinology 122:2508-2517(1988)

PubMed=1366653; DOI=10.1007/BF00365265
Annette Bonhoff, Birgit Gellersen, Karsten R. Held, Heinz G. Bohnet;
Clonal derivatives of a human B-lymphoblastoid cell line producing prolactin -- a cytogenetic characterization.
Cytotechnology 3:43-50(1990)

CLPUB00447
Richard A. Mulivor, Sharon F. Suchy;
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In misc. document) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda; USA (1992)

PubMed=8139288; DOI=10.1016/0145-2126(94)90118-x
Seitetsu Sato, Yoshio Honma, Motoo Hozumi, Yasuhide Hayashi, Yoshinobu Matsuo, Kiyoshi Shibata, Satoshi Omura, Ken-ichiro Hino, Shigeru Tomoyasu, Nobuyoshi Tsuruoka;
Effects of herbimycin A and its derivatives on growth and differentiation of Ph1-positive acute lymphoid leukemia cell lines.
Leuk. Res. 18:221-228(1994)

PubMed=7579375; DOI=10.1182/blood.V86.10.4001.bloodjournal86104001
Catherine Pellat-Deceunynck, Martine Amiot, Francois-Regis Bataille, Ivan Van Riet, Benjamin Van Camp, Paola Omede, Mario Boccadoro;
Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after.
Blood 86:4001-4002(1995)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588
Noriko Hosoya, Akira Hangaishi, Seishi Ogawa, Kiyoshi Miyagawa, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai;
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=10516762; DOI=10.1038/sj.leu.2401510
Hans Gunther Drexler, Wilhelm Gerhard Dirks, Roderick A.F. MacLeod;
False human hematopoietic cell lines: cross-contaminations and misinterpretations.
Leukemia 13:1601-1607(1999)

PubMed=10936422; DOI=10.1016/S0145-2126(99)00195-2
Hans Gunther Drexler, Yoshinobu Matsuo;
Malignant hematopoietic cell lines: in vitro models for the study of multiple myeloma and plasma cell leukemia.
Leuk. Res. 24:681-703(2000)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20454443; DOI=10.1155/2010/904767; PMCID=PMC2861168
Cord C. Uphoff, Sabine A. Denkmann, Klaus G. Steube, Hans Gunther Drexler;
Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines.
J. Biomed. Biotechnol. 2010:904767.1-904767.23(2010)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783
Setsuko Shioda, Fumio Kasai, Ken Watanabe, Kohei Kawakami, Azusa Ohtani, Masashi Iemura, Midori Ozawa, Akemi Arakawa, Noriko Hirayama, Eiko Kawaguchi, Tomoko Tano ...Show all 15 authors... , Sayaka Miyata, Motonobu Satoh, Norio Shimizu, Arihiro Kohara; Show fewer authors
Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.
R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CCL-159
BCRC; 60115
CLS; 302151
Coriell; GM04680
DSMZ; ACC-117
ECACC; 86051302
ICLC; HTL99009
IZSLER; BS TCL 98
JCRB; IFO50025
JCRB; JCRB0024
KCLB; 10159
Cell line databases/resources CLO; CLO_0006703
CLO; CLO_0006705
CLO; CLO_0019036
CLDB; cl2635
CLDB; cl2636
CLDB; cl2637
CLDB; cl4962
CLDB; cl5210
CCRID; 1102HUM-NIFDC00064
Cell_Model_Passport; SIDM00632
Cosmic-CLP; 753563
DepMap; ACH-002247
DSMZCellDive; ACC-117
LINCS_LDP; LCL-1958
Lonza; 9
Anatomy/cell type resources BTO; BTO_0002897
Biological sample resources BioSample; SAMN03471460
BioSample; SAMN03472807
CRISP screens repositories BioGRID_ORCS_Cell_line; 899
Chemistry resources ChEMBL-Cells; CHEMBL3307412
ChEMBL-Targets; CHEMBL614584
GDSC; 753563
PharmacoDB; IM9_656_2019
PubChem_Cell_line; CVCL_1305
Encyclopedic resources Wikidata; Q54897504
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1374572
GEO; GSM1669932
Polymorphism and mutation databases Cosmic; 753563
Cosmic; 888021
Cosmic; 919137
IARC_TP53; 21395
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43