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Analytical Methods and Instrumentation

This document outlines an analytical methods and instrumentation course that covers various topics over 5 units. The units include spectrometry, molecular spectroscopy, NMR and mass spectrometry, separation methods, and electroanalysis and surface microscopy. The course aims to teach students the principles of these analytical techniques and their applications. It will provide knowledge of instrumentation, theory, and data analysis methods in these areas. The course objectives, outlines for each unit, outcomes, textbooks and references are presented.

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Mathavaraja Jeyaraman
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866 views2 pages

Analytical Methods and Instrumentation

This document outlines an analytical methods and instrumentation course that covers various topics over 5 units. The units include spectrometry, molecular spectroscopy, NMR and mass spectrometry, separation methods, and electroanalysis and surface microscopy. The course aims to teach students the principles of these analytical techniques and their applications. It will provide knowledge of instrumentation, theory, and data analysis methods in these areas. The course objectives, outlines for each unit, outcomes, textbooks and references are presented.

Uploaded by

Mathavaraja Jeyaraman
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

OBT751 ANALYTICAL METHODS AND INSTRUMENTATION LTPC

3 00 3

OBJECTIVES:
 To study about the principle of spectrometry & the optical instruments.
 To gain knowledge about the theoretical aspects of molecular spectroscopy.
 To Develop knowledge of NMR & mass spectrometry.
 To comprehend different separation methods are used in analytical.
 To gain Knowledge on different microscopy.

UNIT I SPECTROMETRY 9
Properties of electromagnetic radiation- wave properties – components of optical instruments–
Sources of radiation – wavelength selectors – sample containers – radiation transducers – Signal
process and read outs – signal to noise ratio - sources of noise – Enhancement of signal to noise -
types of optical instruments – Applications.

UNIT II MOLECULAR SPECTROSCOPY 9


Molecular absorption spectrometry – Measurement of Transmittance and Absorbance – Beer’s law –
Instrumentation - Applications -Theory of fluorescence and Phosphorescence –Theory of
Infrared absorption spectrometry – IR instrumentation – Applications – Theory of Raman spectroscopy
– Instrumentation – applications.

UNIT III NMR AND MASS SPECTROMETRY


9
Theory of NMR –– chemical shift- NMR-spectrometers – applications of 1H and 13C NMR-
Molecular mass spectra – ion sources. Mass spectrometer. Applications of molecular mass - Electron
paramagnetic resonance- g values –instrumentation.

UNIT IV SEPARATION METHODS 9


General description of chromatography – Band broadening and optimization of column performance-
Liquid chromatography – Partition chromatography – Adsorption chromatography – Ion
exchange chromatography -size exclusion chromatography- Affinity chromatography- principles of
GC and applications – HPLC- Capillary electrophoresis – Applications.

UNIT V ELECTRO ANALYSIS AND SURFACE MICROSCOPY 9


Electrochemical cells- Electrode potential cell potentials – potentiometry- reference electrode – ion
selective and molecular selective electrodes – Instrument for potentiometric studies – Voltametry –
Cyclic and pulse voltametry- Applications of voltametry . Study of surfaces – Scanning probe
microscopes – AFM and STM.
TOTAL: 45 PERIODS
OUTCOMES:
At the end of the course, the student should be able to:
CO1: Gain knowledge on principle of spectrometry & the optical instruments.
CO2: Able to gain knowledge about the theoretical aspects of molecular spectroscopy.
CO3: Develop knowledge of NMR & mass spectrometry.
CO4: Able to comprehend different separation methods are used in analytical.
CO5: Gain Knowledge on different microscopy.
TEXT BOOKS:
1. Skoog, D.A. F. James Holler, and Stanky, [Link] “Instrumental Methods
of Analysis”.Cengage Learning , 2007.
2. Willard, Hobart, etal., “Instrumental Methods of Analysis”. VIIth Edition, CBS, 1986.
3. Braun, Robert D. “ Introduction to Instrumental Analysis”. Pharma Book Syndicate, 1987.
4. Ewing,G.W. “Instrumental Methods of Chemical Analysis”, Vth Edition, McGraw-Hill, 1985

REFERENCES:
1. Sharma, B.K. “Instrumental Methods of Chemical Analysis : Analytical
Chemistry” GoelPublishing House, 1972.
2. Haven, Mary C., etal., “Laboratory Instrumentation “. IVth Edition, John Wiley, 1995.

CO/POs A B C D E F G H I J K L
CO1     
CO2    
CO3     
CO4      
CO5        

CO/POs A B C D E F G H I J K L
CO1 3 2 2 2
CO2 3 2 2 2 1
CO3 2 3 1 2
CO4 2 1 1 2 2
CO5 1 2 2 2 3 2 3

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