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Absorbance and Concentration Calculations

This document contains calculations to determine the concentration of unknown solutions through absorbance measurements. Five solutions of known Co concentration were created and their absorbance measured. A calibration curve of absorbance vs concentration was generated and used to calculate the concentration of an unknown solution based on its absorbance measurement. The unknown solution was found to have a concentration of 0.129 M.

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Brendan Black
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63 views3 pages

Absorbance and Concentration Calculations

This document contains calculations to determine the concentration of unknown solutions through absorbance measurements. Five solutions of known Co concentration were created and their absorbance measured. A calibration curve of absorbance vs concentration was generated and used to calculate the concentration of an unknown solution based on its absorbance measurement. The unknown solution was found to have a concentration of 0.129 M.

Uploaded by

Brendan Black
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd

Solutions

1
mL Co
mL H2O
Final Volume (mL)
Absorbance
Concentration M

1.15
4.20
5.35
0.175
0.032

Concentration Calculations:
Solution 1
(0.15 M)(1.15
Solution 2
(0.15 M)(2.10
Solution 3
(0.15 M)(3.00
Solution 4
(0.15 M)(4.15
Solution 5
(0.15 M)(5.15

2.10
2.95
5.05
0.346
0.062

mL)=(
mL)=(
mL)=(
mL)=(
mL)=(

?
?
?
?
?

M )(5.35
M )(5.05
M )(5.10
M )(5.20
M) (5.15

3.00
2.10
5.10
0.466
0.088

4.15
1.05
5.20
0.655
0.120

5.15
0.00
5.15
0.824
0.150

mL)
mL)
mL)
mL)
mL)

Continued (Multiplying left side)


(0.1725M/mL)=( ? M )(5.35 mL)
(0..315M/mL)=( ? M )(5.05 mL)
(0.45M/mL)=( ? M )(5.10 mL)
(0.6225M/mL)=( ? M )(5.20 mL)
(0.7725M/mL)=( ? M )(5.15 mL)

Last Calculation. Divide Left side by volume on right to get Concentration. Correct Sig Figs
0.032 = M
0.062 = M
0.088 = M
0.120 = M
0.150 = M
Brendan Black

Unknown

0.706
0.129

ultiplying left side)


)=( ? M )(5.35 mL)
)=( ? M )(5.05 mL)
=( ? M )(5.10 mL)
)=( ? M )(5.20 mL)
)=( ? M )(5.15 mL)

ion. Correct Sig Figs.

Absorbance vs. Concentration


0.9

0.8

f(x) = 5.486302618x - 0.0036395651


R = 0.998910876

0.7

Absorbance

0.6

0.5

Unknown

0.4

.706=5.4863x-.0036
.7096=5.4863x
.129=x

0.3

0.2

0.1

0
0.020

0.040

0.060

0.080

0.100

Concentration

0.120

0.140

0.160

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