Agent John Bishop who has been a well-known character appearing in many incarnations of the franchise since his original introduction in the third season of Teenage Mutant Ninja Turtles (2003) has been gender-swapped and renamed as Josefina Bishop for the series. Much like her male counterpart, she is also part of the Earth Protection Force and also sees all mutants as a danger to the world.
Jackie Chan doesn't reprise his role as splinter from Teenage Mutant Ninja Turtles: Mutant Mayhem.
The first TMNT show that references current real world pop culture.