- Her first acting job was in a Pringles commercial when she was 11 years old.
- Lost out to 2 roles in 2012 to Anne Hathaway, she auditioned for 'The Dark Knight Rises' and 'Les Misérables', both roles went to Hathaway.
- Was considered to play Natasha Romanov/Black Widow in 'Iron Man 2' after Emily Blunt dropped out, but Scarlett Johansson was cast instead.
- Won an acting scholarship to the Young Actor's Space at 11 years old.
- Auditioned for the role of Agent Fields in 'Quantum of Solace', the role went to Gemma Arterton.
- Once wanted to become a musical star after winning a karaoke contest when she was aged eight.
- Jessica's paternal great-grandfather, Edward Biel, was the son of Moritz Biel and Otillie Morskovitz, who were Jewish emigrants from Zilina (then in the Kingdom of Hungary, now in Slovakia). She discovered this lineage on the show Who Do You Think You Are? (2010). Edward's wife, Jessica's great-grandmother Carrie Rasmussen, was the daughter of Danish parents. Jessica's other ancestry includes English, German, Swiss-German, Scottish, and distant Welsh, Swedish, and French, roots.
- Her father ran an Outward Bound school and worked as an international business consultant.
- Has a brother named Justin Biel.
- Named a Global Brand Ambassador for Revlon [May 4, 2009].
- She collects vintage glasses with no lenses.
- Discovered at the 1994 IMTA Los Angeles convention.
- Attended Tufts University for three semesters.
- Auditioned for the role of Angela Hayes in American Beauty (1999).
- Daughter of Kimberly (Kim) Biel (née Conroe) and Jonathan Edward Biel, an entrepreneur.
- Has two sons with her husband Justin Timberlake Silas Randall and Phineas.
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