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Highlighted in Zika Virus Collection

Zika virus impairs growth in human neurospheres and brain organoids

, , , , , , , , ,
1 Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
2 D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil
3 Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
4 Department of Biochemistry and Tissue Biology / Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil
DOI
10.7287/peerj.preprints.1817v3
Published
Accepted
Subject Areas
Cell Biology, Developmental Biology, Neuroscience
Keywords
zika virus, cerebral organoids, cell death, microcephaly, stem cells
Copyright
© 2016 Garcez et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Garcez PP, Loiola EC, Madeiro da Costa RF, Higa L, Trindade P, Delvecchio R, Nascimento JM, Brindeiro RM, Tanuri A, Rehen SK. 2016. Zika virus impairs growth in human neurospheres and brain organoids. PeerJ Preprints 4:e1817v3

Abstract

Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased dramatically in Brazil; however, causality between the widespread epidemic and malformations in fetal brains has not been confirmed. Here, we examine the effects of ZIKV infection in human neural stem cells growing as neurospheres and cerebral organoids. Using immunocytochemistry and electron microscopy, we show that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and cerebral organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development.

Author Comment

This version shows a more accurate scale bar on figure 3.

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Patricia P Garcez conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Erick C Loiola conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Rodrigo F Madeiro da Costa conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Luiza Higa conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Pablo Trindade conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Rodrigo Delvecchio conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Juliana M Nascimento conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Rodrigo M Brindeiro conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Almicar Tanuri conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Stevens K Rehen conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All protocols and procedures were approved by the institutional research ethics committee of Hospital Copa D'Or (CEPCOPADOR) under # 727.269.

Data Deposition

The following information was supplied regarding data availability:

The research in this article did not generate any raw data.

Funding

Funds (not specifically for Zika virus studies) were provided by the Brazilian Development Bank (BNDES); Funding Authority for Studies and Projects (FINEP); and fellowships from the National Council of Scientific and Technological Development (CNPq); Foundation for Research Support in the State of Rio de Janeiro (FAPERJ); São Paulo Research Foundation (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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